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Abstract

Three Gram-positive, rod-shaped, oxidase-negative, non-spore-forming, non-motile bacteria (KSS-3Se, KSS-4Se and KSS-10Se) were isolated from a coolant lubricant. 16S rRNA gene sequence analyses revealed almost identical sequences, with only a few (<10 positions) differences for these three isolates. Comparisons showed the highest similarities to NCTC 11862 (97.6 % similarity with strain KSS-3Se). Similarities with other established species were lower than 97.0 %. Chemotaxonomic data studied for strain KSS-3Se [polar lipids – major compounds phosphatidylglycerol and an unknown glycolipid, moderate amounts of phosphatidylinositol and diphosphatidylglycerol; polyamines (small amounts) – major compounds spermidine and spermine; quinones – significant amounts of menaquinones MK-9(H), MK-8(H) and MK-7(H); and major fatty acids – tuberculostearic acid (10-methyl C), C and C 9] were congruent with those reported for . The strain showed differences in phenotype from . DNA–DNA hybridizations between KSS-3Se and DSM 20521 yielded a relatedness of 22.9 % (20.4 % in the reciprocal assay). From these results, it is evident that the organisms represent a novel species, for which the name sp. nov. is proposed (type strain KSS-3Se =DSM 45231 =CCUG 56567 =CCM 7546).

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2009-05-01
2019-12-16
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Supplements

vol. , part 5, pp. 1112 - 1115

Phylogenetic analysis based on 16S rRNA gene sequences available from the EMBL database showing the position of strains KSS-3Se , KSS-4Se and KSS-10Se, including an extended selection of reference sequences.

Biochemical characteristics of sp. nov. and the most similar species (on the basis of 16S rRNA gene sequence similarity) obtained with the API Coryne and API ZYM systems

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