Two Gram-variable, aerobic, motile, rod-shaped, endospore-forming bacterial strains, M4-2 and M4-1, were isolated from soil samples collected from Oblast Magadan, Russian Far East, as micro-organisms antagonistic to the psychrophilic phytopathogenic fungus . Strains M4-2 and M4-1 were identified as members of the genus by phenotypic and phylogenetic analyses based on 16S rRNA gene sequences. The strains contained anteiso-C as the major fatty acid (63.0–64.7 %) and MK-7 as the major isoprenoid quinone. The DNA G+C contents were 42.8 and 41.7 mol%, respectively. 16S rRNA gene sequence analysis showed that strains M4-2 and M4-1 exhibited high similarities with DSM 2 (99.5 and 99.7 %, respectively) and LMG 22078 (99.4 and 99.5 %, respectively). There were no clear differences in the phenotypic characteristics and chemotaxonomic and phylogenetic data between the novel isolates and DSM 2. DNA–DNA hybridization experiments between strain M4-2 and DSM 2 and LMG 22078 revealed reassociation values of 56 and 49 %, respectively. Multilocus sequence analysis confirmed the differences between the new isolates and reference strains that were observed with the DNA–DNA hybridization studies. On the basis of the results described above, it is proposed that the isolates represent a novel subspecies of , subsp. subsp. nov. The type strain is M4-2 (=JCM 14954=NCIMB 14397).


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vol. , part 8, pp. 2074 - 2079

DNA–DNA relatedness among strains examined in this study.

Macromorphology of co-cultured with subsp. subsp. nov. M4-2 (a) and alone (b) after incubation at 10°C for 30 days.

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