Two novel micro-organisms, designated strains YIT 10443T and YIT 10738, were isolated from airag, a traditional fermented mare's milk from Mongolia. The two strains were Gram-positive-staining, non-motile, asporogenous, catalase-negative, facultatively anaerobic rods of various shapes. Comparative analyses of 16S rRNA and ClpC ATPase (clpC) gene sequences and the presence of fructose-6-phosphate phosphoketolase (F6PPK) demonstrated that the novel strains were members of the genus Bifidobacterium. On the basis of 16S rRNA gene sequence similarity, the type strains of Bifidobacterium minimum (96.6 %) and Bifidobacterium psychraerophilum (95.7 %) were the closest neighbours of the novel strains, and DNA–DNA reassociation values with these strains were found to be lower than 15 %. The phenotypic and genotypic features demonstrated that the two strains represent a single, novel Bifidobacterium species, for which the name Bifidobacterium mongoliense sp. nov. is proposed. The type strain is YIT 10443T (=JCM 15461T =DSM 21395T).
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Supplementary Fig. S1. RAPD-PCR fingerprinting of
strains YIT 10443
T(lanes 1) and YIT 10738 (lanes 2). Three primers
with random sequences (A, 5′-CCGCAGCCA A-3′; B,
5′-AACGCGCAAC-3′; C, 5′-GCGGAAATAG-3′)
were used. Lane M, 100 bp ladder DNA size marker (Seegene).
Supplementary Fig. S2. Phylogenetic tree based on 16S
rRNA gene sequences constructed by using the maximum-likelihood
method based on comparison of approximately 1460 positions.
[PDF](63 KB)
Supplementary Fig. S3. Scanning electron micrographs of
cells of
Bifidobacterium mongoliense sp. nov. YIT 10443
T(a, b) and
B. mongoliense sp. nov. YIT 10738 (c, d). (a, c) Cells
grown in modified GAM broth overnight at 30 °C under
anaerobic conditions; (b, d) cells grown on modified GAM agar
plate at 30 °C for 3 days under aerobic conditions. Bars,
1 µm.