1887

Abstract

Two novel micro-organisms, designated strains YIT 10443 and YIT 10738, were isolated from airag, a traditional fermented mare's milk from Mongolia. The two strains were Gram-positive-staining, non-motile, asporogenous, catalase-negative, facultatively anaerobic rods of various shapes. Comparative analyses of 16S rRNA and ClpC ATPase () gene sequences and the presence of fructose-6-phosphate phosphoketolase (F6PPK) demonstrated that the novel strains were members of the genus . On the basis of 16S rRNA gene sequence similarity, the type strains of (96.6 %) and (95.7 %) were the closest neighbours of the novel strains, and DNA–DNA reassociation values with these strains were found to be lower than 15 %. The phenotypic and genotypic features demonstrated that the two strains represent a single, novel species, for which the name sp. nov. is proposed. The type strain is YIT 10443 (=JCM 15461 =DSM 21395).

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2009-06-01
2019-12-11
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RAPD-PCR fingerprinting of strains YIT 10443 (lanes 1) and YIT 10738 (lanes 2). Three primers with random sequences (A, 5′-CCGCAGCCA A-3′; B, 5′-AACGCGCAAC-3′; C, 5′-GCGGAAATAG-3′) were used. Lane M, 100 bp ladder DNA size marker (Seegene).

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Phylogenetic tree based on 16S rRNA gene sequences constructed by using the maximum-likelihood method based on comparison of approximately 1460 positions. [PDF](63 KB)

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Scanning electron micrographs of cells of sp. nov. YIT 10443 (a, b) and sp. nov. YIT 10738 (c, d). (a, c) Cells grown in modified GAM broth overnight at 30 °C under anaerobic conditions; (b, d) cells grown on modified GAM agar plate at 30 °C for 3 days under aerobic conditions. Bars, 1 µm.

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