Strain VC-230 was isolated from homemade vermicompost produced from kitchen waste. The isolate was a Gram-negative-staining, catalase- and oxidase-positive, motile rod-shaped bacterium able to grow at 15–37 °C and pH 6–8. On the basis of 16S rRNA gene sequence analysis, strain VC-230 was determined to belong to the family by its clustering with type strains of the genus , with ATCC 33790 (97.7 %) and DSM 11019 (97.4 %) as its closest neighbours. The polar lipid pattern, the presence of spermidine and ubiquinone 10, the predominance of the cellular fatty acids C 7/9/12, C 7 and C and the G+C content of the genomic DNA supported the affiliation of this organism to the genus . The phylogenetic, chemotaxonomic, phenotypic and DNA–DNA hybridization analyses verify that strain VC-230 represents a novel species, for which the name sp. nov. is proposed. The type strain is VC-230 (=CCUG 55809 =DSM 21299).


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  1. Barreiros, L., Nogales, B., Manaia, C. M., Ferreira, A. C. S., Pieper, D. H., Reis, M. A. & Nunes, O. C.(2003). A novel pathway for mineralization of the thiocarbamate herbicide molinate by a defined bacterial mixed culture. Environ Microbiol 5, 944–953.[CrossRef] [Google Scholar]
  2. Busse, H.-J., Kämpfer, P. & Denner, E. M. B.(1999). Chemotaxonomic characterisation of Sphingomonas. J Ind Microbiol Biotechnol 23, 242–251.[CrossRef] [Google Scholar]
  3. Epstein, E.(1997).The Science of Composting. Boca Raton, FL: CRC Press.
  4. Euzéby, J. P.(1997). List of bacterial names with standing in nomenclature: a folder available on the Internet. Int J Syst Bacteriol 47, 590–592 http://www.bacterio.cict.fr.[CrossRef] [Google Scholar]
  5. Ferreira da Silva, M., Tiago, I., Veríssimo, A., Boaventura, A. R., Nunes, O. C. & Manaia, C. M.(2006). Antibiotic resistance of enterococci and related bacteria in an urban wastewater treatment plant. FEMS Microbiol Ecol 55, 322–329.[CrossRef] [Google Scholar]
  6. Ferreira da Silva, M., Vaz-Moreira, I., Gonzalez-Pajuelo, M., Nunes, O. C. & Manaia, C. M.(2007). Antimicrobial resistance patterns in Enterobacteriaceae isolated from an urban wastewater treatment plant. FEMS Microbiol Ecol 60, 166–176.[CrossRef] [Google Scholar]
  7. Fujii, K., Urano, N., Ushio, H., Satomi, M. & Kimura, S.(2001).Sphingomonas cloacae sp. nov., a nonylphenol-degrading bacterium isolated from wastewater of a sewage-treatment plant in Tokyo. Int J Syst Evol Microbiol 51, 603–610. [Google Scholar]
  8. Hamana, K., Sakane, T. & Yokota, A.(1994). Polyamine analysis of the genera Aquaspirillum, Magnetospirillum, Oceanospirillum and Spirillum. J Gen Appl Microbiol 40, 75–82.[CrossRef] [Google Scholar]
  9. Jukes, T. H. & Cantor, C. R.(1969). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  10. Manaia, C. M., Nogales, B., Weiss, N. & Nunes, O. C.(2004).Gulosibacter molinativorax gen. nov., sp. nov., a molinate-degrading bacterium, and classification of ‘Brevibacterium helvolum’ DSM 20419 as Pseudoclavibacter helvolus gen. nov., sp. nov. Int J Syst Evol Microbiol 54, 783–789.[CrossRef] [Google Scholar]
  11. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  12. Murray, R. G. E., Doetsch, R. N. & Robinow, F.(1994). Determinative and cytological light microscopy. In Methods for General and Molecular Bacteriology, pp. 21–41. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  13. Nohynek, L. J., Suhonen, E. L., Nurmiaho-Lassila, E. L., Hantula, J. & Salkinoja-Salonen, M.(1995). Description of four pentachlorophenol-degrading bacterial strains as Sphingomonas chlorophenolica sp. nov. Syst Appl Microbiol 18, 527–538.[CrossRef] [Google Scholar]
  14. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  15. Stackebrandt, E. & Ebers, J.(2006). Taxonomic parameters revisited: tarnished gold standards. Microbiol Today 33, 152–155. [Google Scholar]
  16. Takeuchi, M., Hamana, K. & Hiraishi, A.(2001). Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. Int J Syst Evol Microbiol 51, 1405–1417. [Google Scholar]
  17. Tindall, B. J.(1989). Fully saturated menaquinones in the archaebacterium Pyrobaculum islandicum. FEMS Microbiol Lett 60, 251–254.[CrossRef] [Google Scholar]
  18. Urdiain, M., López-López, A., Gonzalo, C., Busse, H.-J., Langer, S., Kämpfer, P. & Rosselló-Móra, R.(2008). Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov., a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium, Rhodobium orientis and Rhodobium gokarnense. Syst Appl Microbiol 31, 339–351.[CrossRef] [Google Scholar]
  19. Vaz-Moreira, I., Nobre, M. F., Nunes, O. C. & Manaia, C. M.(2007).Gulbenkiania mobilis gen. nov., sp. nov., isolated from treated municipal wastewater. Int J Syst Evol Microbiol 57, 1108–1112.[CrossRef] [Google Scholar]
  20. Vaz-Moreira, I., Silva, E., Manaia, C. M. & Nunes, O. C.(2008). Diversity of bacterial isolates from commercial and homemade composts. Microb Ecol 55, 714–722.[CrossRef] [Google Scholar]
  21. Yabuuchi, E., Kosako, Y., Fugiwara, N., Naka, T., Matsunaga, I., Ogura, H. & Kobayashi, K.(2002). Emendation of the genus Sphingomonas Yabuuchi et al. 1990 and junior objective synonymy of the species of three genera, Sphingobium, Novosphingobium and Sphingopyxis, in conjunction with Blastomonas ursincola. Int J Syst Evol Microbiol 52, 1485–1496.[CrossRef] [Google Scholar]
  22. Zipper, C., Nickel, K., Angst, W. & Kohler, H. P. E.(1996). Complete microbial degradation of both enantiomers of the chiral herbicide mecoprop [(RS)-2-(4-chloro-2-methylphenoxy)propionic acid] in an enantioselective manner by Sphingomonas herbicidovorans sp. nov. Appl Environ Microbiol 62, 4318–4322. [Google Scholar]

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