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Abstract

A psychrophilic, Gram-negative, dark orange-pigmented bacterium, designated CL-AP4, was isolated from a culture of the green alga obtained from the Southern Ocean. Strain CL-AP4 grew optimally at 10 °C, in the presence of 3–4 % sea salts and at pH 8. 16S rRNA gene sequence analysis revealed that strain CL-AP4 belonged to the family , with KOPRI 20941 as its closest relative (97.2 % similarity). A number of chemotaxonomic characteristics supported affiliation of strain CL-AP4 with the genus , i.e. iso-C (17.2 %), iso-C (16.8 %) and iso-C 3-OH (14.9 %) were the dominant fatty acids, MK-6 was the major menaquinone and the DNA G+C content was 37.1 mol%. DNA–DNA relatedness between CL-AP4 and KOPRI 20941 was only 10 %, suggesting that they are genomically distinct species. In addition, strain CL-AP4 differed phenotypically from in its optimum growth temperature, its ability to hydrolyse starch, Tween 40 and Tween 80, and production of certain enzymes. On the basis of the results of the polyphasic analysis, strain CL-AP4 was classified in the genus as belonging to a novel species, for which the name sp. nov. is proposed; the type strain is CL-AP4 (=KCCM 90069=JCM 15445).

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2009-06-01
2019-12-15
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vol. , part 6, pp. 1455 – 1459

Comparison of the cellular fatty acid contents of strain CL-AP4 and phylogenetically related species in the genus [ PDF] (147 KB)



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