%0 Journal Article %A Peng, Guixiang %A Zhang, Wu %A Luo, Huifen %A Xie, Hongwei %A Lai, Weihao %A Tan, Zhiyuan %T Enterobacter oryzae sp. nov., a nitrogen-fixing bacterium isolated from the wild rice species Oryza latifolia %D 2009 %J International Journal of Systematic and Evolutionary Microbiology, %V 59 %N 7 %P 1650-1655 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.005967-0 %I Microbiology Society, %X Twelve facultatively anaerobic, endophytic diazotrophs were isolated from surface-sterilized roots of the wild rice species Oryza latifolia and characterized by phenotypic and molecular methods. Six isolates were grouped together as group A by phenotypic characters, and this grouping was confirmed by SDS-PAGE whole-cell protein patterns and insertion sequence-based PCR (IS-PCR) methods. Phylogenetic analysis of the 16S rRNA gene sequence indicated that group A, represented by strain Ola 51T, is closely related to Enterobacter radicincitans D5/23T (98.9 % similarity, except that E. radicincitans D5/23T has a 70 bp insertion) and Enterobacter cloacae (98.0 % similarity to the type strain). rpoB gene sequence analysis also showed strain Ola 51T has the highest sequence similarity to E. radicincitans DSM 16656T (98.3 %), but supported the distinct position. Biological and biochemical tests, protein patterns, genomic DNA fingerprinting, antibiotic resistance and comparison of cellular fatty acids showed differences among group A, E. radicincitans DSM 16656T and E. cloacae ATCC 13047T. DNA–DNA hybridization distinguished strain Ola 51T from closely phylogenetically related Enterobacter species. Based on these data, the novel species Enterobacter oryzae sp. nov. is proposed, with strain Ola 51T (=LMG 24251T =CGMCC 1.7012T) as the type strain. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.005967-0