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Volume 59, Issue 7

sp. nov., a nitrogen-fixing bacterium isolated from the wild rice species Free

Abstract

Twelve facultatively anaerobic, endophytic diazotrophs were isolated from surface-sterilized roots of the wild rice species and characterized by phenotypic and molecular methods. Six isolates were grouped together as group A by phenotypic characters, and this grouping was confirmed by SDS-PAGE whole-cell protein patterns and insertion sequence-based PCR (IS-PCR) methods. Phylogenetic analysis of the 16S rRNA gene sequence indicated that group A, represented by strain Ola 51, is closely related to D5/23 (98.9 % similarity, except that D5/23 has a 70 bp insertion) and (98.0 % similarity to the type strain). gene sequence analysis also showed strain Ola 51 has the highest sequence similarity to DSM 16656 (98.3 %), but supported the distinct position. Biological and biochemical tests, protein patterns, genomic DNA fingerprinting, antibiotic resistance and comparison of cellular fatty acids showed differences among group A, DSM 16656 and ATCC 13047. DNA–DNA hybridization distinguished strain Ola 51 from closely phylogenetically related species. Based on these data, the novel species sp. nov. is proposed, with strain Ola 51 (=LMG 24251 =CGMCC 1.7012) as the type strain.

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2009-07-01
2024-04-19
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Enterobacter oryzae sp. nov., a nitrogen-fixing bacterium isolated from the wild rice species Oryza latifolia
Int J Syst Evol Microbiol 59, 1650 (2009); https://doi.org/10.1099/ijs.0.005967-0
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Transmission electron micrograph of a cell of strain Ola 51 showing a rod with the flagellar structure. Bar, 0.2 µm.

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IS-PCR fingerprinting patterns of diazotrophic isolates of group A from the wild rice species and closely related species. M, Standard DNA marker; lanes 1–9, ATCC 13048 , ATCC 13047 , DSM 16656 and group A isolates Ola 51 , Ola 10, Ola 50, Ola 12, Ola 01 and Ola 28.

IMAGE

Whole-cell protein SDS-PAGE patterns of diazotrophic isolates of group A from and closely related reference strains. M, Molecular-mass standards; lanes 1–8, ATCC 13048 , ATCC 13047 and group A isolates Ola 51 , Ola 10, Ola 50, Ola 12, Ola 01 and Ola 28. Arrows indicate intense protein bands that differ between ATCC 13047 and the diazotrophic isolates.

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Neighbour-joining dendrogram based on gene sequences of sp. nov. Ola 51 and closely related species. [PDF](30 KB)

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[PDF file of Supplementary Tables S1-S3](98 KB)

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