1887

Abstract

A Gram-positive, slightly acid–alcohol-fast, carbon monoxide-oxidizing bacterium, strain Y2, was isolated from a soil sample collected from a roadside in Seoul, Korea. On the basis of 16S rRNA gene sequence comparative analyses, strain Y2 was shown to belong to the genus and was most closely related to DSM 44234 (GenBank accession no. AY238514; 99.8 %). The predominant fatty acids were C 9 and C. The cell-wall peptidoglycan of strain Y2 contained -diaminopimelic acid as the diagnostic diamino acid. Strain Y2 contained galactose and arabinose as the whole cell sugars. The DNA G+C content was 77 mol%. The DNA–DNA relatedness value between strain Y2 and DSM 44234 was 62.7 %. Based on the combination of the carbon source utilization pattern, fatty acid profile, cell-wall chemotype, DNA G+C content and DNA–DNA hybridization experiments, it is proposed that strain Y2 (=KCCM 42885=JCM 15482) represents the type strain of a novel species, sp. nov.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.005959-0
2009-06-01
2019-12-05
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/6/1541.html?itemId=/content/journal/ijsem/10.1099/ijs.0.005959-0&mimeType=html&fmt=ahah

References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. ( 1995; ). Current Protocols in Molecular Biology. Boston, MA: Wiley.
  2. Collins, M. D., Smida, J., Dorsch, M. & Stackebrandt, E. ( 1988; ). Tsukamurella gen. nov., harboring Corynebacterium paurometabolum and Rhodococcus aurantiacus. Int J Syst Bacteriol 38, 385–391.[CrossRef]
    [Google Scholar]
  3. Ebersole, L. L. ( 1992; ). Acid-fast staining procedures. In Clinical Microbiology Procedures Handbook, vol 1, pp. 3.5.1–3.5.11. Edited by H. D. Isenberg. Washington, DC: American Society for Microbiology.
  4. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  5. Goodfellow, M. & O'Donnell, A. G. ( 1989; ). Search and discovery of industrially significant actinomycetes. In Microbial Products: New Approaches, pp. 343–383. Edited by S. Baumberg, P. M. Rhodes & I. S. Hunter. Cambridge: Cambridge University Press.
  6. Goodfellow, M., Stainsby, F. M., Davenport, R., Chun, J. & Curtis, T. P. ( 1998; ). Activated sludge foaming: the true extent of actinomycete diversity. Water Sci Technol 37, 511–519.[CrossRef]
    [Google Scholar]
  7. Gordon, R. E. ( 1966; ). Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol 45, 355–364.[CrossRef]
    [Google Scholar]
  8. Gordon, R. E. & Mihm, J. M. ( 1957; ). A comparative study of some strains received as nocardiae. J Bacteriol 73, 15–27.
    [Google Scholar]
  9. Gordon, R. E. & Smith, M. M. ( 1955; ). Proposed group of characters for the separation of Streptomyces and Nocardia. J Bacteriol 69, 147–150.
    [Google Scholar]
  10. Kattar, M. M., Cookson, B. T., Carlson, L. D., Stiglich, S. K., Schwartz, M. A., Nguyen, T. T., Daza, R., Wallis, C. K., Yarfitz, S. L. & Coyle, M. B. ( 2001; ). Tsukamurella strandjordae sp. nov., a proposed new species causing sepsis. J Clin Microbiol 39, 1467–1476.[CrossRef]
    [Google Scholar]
  11. Kim, Y. M. & Hegeman, G. D. ( 1981; ). Purification and some properties of carbon monoxide dehydrogenase from Pseudomonas carboxydohydrogena. J Bacteriol 148, 904–911.
    [Google Scholar]
  12. King, G. M. ( 2003; ). Molecular and culture-based analyses of aerobic carbon monoxide oxidizer diversity. Appl Environ Microbiol 69, 7257–7265.[CrossRef]
    [Google Scholar]
  13. Kraut, M., Hugendieck, I., Herwig, S. & Meyer, O. ( 1989; ). Homology and distribution of CO dehydrogenase structural genes in carboxydotrophic bacteria. Arch Microbiol 152, 335–341.[CrossRef]
    [Google Scholar]
  14. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). MEGA3: Integrated software for molecular evolutionary analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  15. Lane, D. J. ( 1991; ). 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by E. Stackebrandt & M. Goodfellow. London: Wiley.
  16. Mesbah, M., Premachandran, U. & Whiteman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  17. Miller, L. T. ( 1982; ). Single derivatization method for routine analysis of bacterial whole-cell fatty acid methyl esters, including hydroxy acids. J Clin Microbiol 16, 584–586.
    [Google Scholar]
  18. Nam, S.-W., Chun, J., Kim, S., Kim, W., Zakrzewska-Czerwinska, J. & Goodfellow, M. ( 2003; ). Tsukamurella spumae sp. nov., a novel actinomycete associated with foaming in activated sludge plants. Syst Appl Microbiol 26, 367–375.[CrossRef]
    [Google Scholar]
  19. Nam, S.-W., Kim, W., Chun, J. & Goodfellow, M. ( 2004; ). Tsukamurella pseudospumae sp. nov., a novel actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 54, 1209–1212.[CrossRef]
    [Google Scholar]
  20. Nicholas, R. A., Khan, L. A., Houshaymi, B., Miles, R. J., Ayling, R. D., Hotzel, H. & Sachse, K. ( 2002; ). Close genetic and phenotypic relatedness between Mycoplasma ovine/caprine serogroup 11 and Mycoplasma bovigenitalium. Syst Appl Microbiol 25, 396–402.[CrossRef]
    [Google Scholar]
  21. Olson, J. B., Harmody, D. K., Bej, A. K. & McCarthy, P. J. ( 2007; ). Tsukamurella spongiae sp. nov., a novel actinomycete isolated from a deep-water marine sponge. Int J Syst Evol Microbiol 57, 1478–1481.[CrossRef]
    [Google Scholar]
  22. Paliwal, D. K. & Randhawa, H. S. ( 1977; ). Rapid method for detection of urea hydrolysis by yeasts. Appl Environ Microbiol 33, 219–220.
    [Google Scholar]
  23. Park, S. W., Hwang, E. H., Park, H., Kim, J. A., Heo, J. H., Lee, K. H., Song, T., Kim, E., Ro, Y. T. & other authors ( 2003; ). Growth of mycobacteria on carbon monoxide and methanol. J Bacteriol 185, 142–147.[CrossRef]
    [Google Scholar]
  24. Park, S. W., Park, S. T., Lee, J. E. & Kim, Y. M. ( 2008; ). Pseudonocardia carboxydivorans sp. nov., a carbon monoxide-oxidizing actinomycete, and an emended description of the genus Pseudonocardia. Int J Syst Evol Microbiol 58, 2475–2478.[CrossRef]
    [Google Scholar]
  25. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  26. Sasser, M. ( 1990; ). Identification of bacteria through fatty acid analysis. In Methods in Phytobacteriology, pp. 199–204. Edited by Z. Klement, K. Rudolph & D. C. Sands. Budapest: Akademiai Kiado.
  27. Sonesson, A., Larsson, L., Fox, A., Westerdahl, G. & Odham, G. ( 1988; ). Determination of environmental levels of peptidoglycan and lipopolysaccharide using gas chromatography with negative-ion chemical-ionization mass spectrometry utilizing bacteria amino acids and hydroxy fatty acids as biomarkers. J Chromatogr 431, 1–15.[CrossRef]
    [Google Scholar]
  28. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  29. Yassin, A. F., Rainey, F. A., Brzezinka, H., Burghardt, J., Lee, H. J. & Schaal, K. P. ( 1995; ). Tsukamurella inchonensis sp. nov. Int J Syst Bacteriol 45, 522–527.[CrossRef]
    [Google Scholar]
  30. Yassin, A. F., Rainey, F. A., Brzezinka, H., Burghardt, J., Rifai, M., Seifert, P., Feldmann, K. & Schaal, K. P. ( 1996; ). Tsukamurella pulmonis sp. nov. Int J Syst Bacteriol 46, 429–436.[CrossRef]
    [Google Scholar]
  31. Yassin, A. F., Rainey, F. A., Burghardt, J., Brzezinka, H., Schmitt, S., Seifert, P., Zimmermann, O., Mauch, H., Gierth, D. & other authors ( 1997; ). Tsukamurella tyrosinosolvens sp. nov. Int J Syst Bacteriol 47, 607–614.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.005959-0
Loading
/content/journal/ijsem/10.1099/ijs.0.005959-0
Loading

Data & Media loading...

Supplements

vol. , part 6, pp. 1541 - 1544

Comparison of the fatty acid contents (%) of strain Y2 with other closely related species of the genus . [ PDF] 44 KB



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error