The taxonomic position of the cholesterol-degrading strain Chol-3, isolated from a sewage sludge sample, was clarified using a polyphasic taxonomic approach. Phylogenetic analysis of its 16S rRNA gene sequence, whole-cell fatty acid profile and mycolic acid composition revealed that this isolate is a member of the genus with the species , and being the nearest phylogenetic neighbours. The results of DNA–DNA hybridization against its phylogenetically closest neighbours as well as the results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Chol-3 from the other species with validly published names. Strain Chol-3 therefore merits recognition as a member of a novel species within the genus , for which the name sp. nov. is proposed. The type strain is Chol-3 (=CECT 7408 =DSM 45229).


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  1. Arenskötter, M., Bröker, D. & Steinbüchel, A.(2004). Biology of the metabolically diverse genus Gordonia. Appl Environ Microbiol 70, 3195–3204.[CrossRef] [Google Scholar]
  2. Blaschke, A. J., Bender, J., Byington, C. L., Korgenski, K., Daly, J., Petti, C. A., Pavia, A. T. & Ampofo, K.(2007).Gordonia species: emerging pathogens in pediatric patients that are identified by 16S ribosomal RNA gene sequencing. Clin Infect Dis 45, 483–486.[CrossRef] [Google Scholar]
  3. Bruce, J.(1996). Automated system rapidly identifies and characterizes microorganisms in food. Food Technol 50, 77–81. [Google Scholar]
  4. Carr, E. L., Eales, K. L. & Seviour, R. J.(2006). Substrate uptake by Gordonia amarae in activated sludge foams by FISH-MAR. Water Sci Technol 54 (1), 39–45. [Google Scholar]
  5. Chatterjee, S. & Dutta, T. K.(2003). Metabolism of butyl benzyl phthalate by Gordonia sp. strain MTCC 4818. Biochem Biophys Res Commun 309, 36–43.[CrossRef] [Google Scholar]
  6. Clark, C. G.(1997). Riboprinting: a general tool for the study of genetic diversity in microorganisms. J Eukaryot Microbiol 44, 277–283.[CrossRef] [Google Scholar]
  7. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  8. Goodfellow, M. & Maldonado, L. A.(2006). The families Dietziaceae, Gordoniaceae, Nocardiaceae and Tsukamurellaceae. In The Prokaryotes, 3rd edn, vol. 3, pp. 843–888. Edited by F. Dworkin, S. Falkow, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  9. Goodfellow, M., Alderson, G. & Chun, J.(1998). Rhodococcal systematics: problems and developments. Antonie van Leeuwenhoek 74, 3–20.[CrossRef] [Google Scholar]
  10. Goodfellow, M., Isik, K. & Yates, E.(1999). Actinomycete systematics: an unfinished synthesis. Nova Acta Leopold 312, 47–82. [Google Scholar]
  11. Gorontzy, T., Drzyzga, O., Kahl, M. W., Bruns-Nagel, D., Breitung, J., von Löw, E. & Blotevogel, K. H.(1994). Microbial degradation of explosives and related compounds. Crit Rev Microbiol 20, 265–284.[CrossRef] [Google Scholar]
  12. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  13. Iida, S., Taniguchi, H., Kageyama, A., Yazawa, K., Chibana, H., Murata, S., Nomura, F., Kroppenstedt, R. M. & Mikami, Y.(2005).Gordonia otitidis sp. nov., isolated from a patient with external otitis. Int J Syst Evol Microbiol 55, 1871–1876.[CrossRef] [Google Scholar]
  14. Iwahori, K., Tokutomo, T., Imbata, N. & Fujita, M.(2001). Formation of stable foam by the cells and culture supernatant of Gordonia (Nocardia) amarae. J Biosci Bioeng 92, 77–79.[CrossRef] [Google Scholar]
  15. Kageyama, A., Iida, S., Yazawa, K., Kudo, T., Suzuki, S., Koga, T., Saito, H., Inagawa, H., Wada, A. & other authors(2006).Gordonia araii sp. nov. and Gordonia effusa sp. nov., isolated from patients in Japan. Int J Syst Evol Microbiol 56, 1817–1821.[CrossRef] [Google Scholar]
  16. Kim, S. B., Brown, R., Oldfield, C., Gilbert, S. C. & Goodfellow, M.(1999).Gordonia desulfuricans sp. nov., a benzothiophene-desulphurizing actinomycete. Int J Syst Bacteriol 49, 1845–1851.[CrossRef] [Google Scholar]
  17. Kim, S. B., Brown, R., Oldfield, C., Gilbert, S. C., Iliarionov, S. & Goodfellow, M.(2000).Gordonia amicalis sp. nov., a dibenzothiophene-desulphurizing actinomycete. Int J Syst Evol Microbiol 50, 2031–2036.[CrossRef] [Google Scholar]
  18. Kim, K. K., Lee, C. S., Kroppenstedt, R. M., Stackebrandt, E. & Lee, S. T.(2003).Gordonia sihwensis sp. nov., a novel nitrate-reducing bacterium isolated from a wastewater-treatment bioreactor. Int J Syst Evol Microbiol 53, 1427–1433.[CrossRef] [Google Scholar]
  19. Kummer, C., Schumann, P. & Stackebrandt, E.(1999).Gordonia alkanivorans sp. nov., isolated from tar-contaminated soil. Int J Syst Bacteriol 49, 1513–1522.[CrossRef] [Google Scholar]
  20. Lechevalier, M. P. & Lechevalier, H.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  21. Lee, J. J., Rhee, S. K. & Lee, S. T.(2001). Degradation of 3-methylpyridine and 3-ethylpyridine by Gordonia nitida LE31. Appl Environ Microbiol 67, 4342–4345.[CrossRef] [Google Scholar]
  22. Le Roes, M., Goodwin, C. M. & Meyers, P. R.(2008).Gordonia lacunae sp. nov., isolated from an estuary. Syst Appl Microbiol 31, 17–23.[CrossRef] [Google Scholar]
  23. Linos, A., Steinbüchel, A., Spröer, C. & Kroppenstedt, R. M.(1999).Gordonia polyisoprenivorans sp. nov., a rubber-degrading actinomycete isolated from an automobile tyre. Int J Syst Bacteriol 49, 1785–1791.[CrossRef] [Google Scholar]
  24. Luo, H., Gu, Q., Xie, J., Hu, C., Liu, Z. & Huang, Y.(2007).Gordonia shandongensis sp. nov., isolated from soil in China. Int J Syst Evol Microbiol 57, 605–608.[CrossRef] [Google Scholar]
  25. Maldonado, L. A., Stainsby, F. M., Ward, A. C. & Goodfellow, M.(2003).Gordonia sinesedis sp. nov., a novel soil isolate. Antonie van Leeuwenhoek 83, 75–80.[CrossRef] [Google Scholar]
  26. Miller, L. & Berger, T.(1985).Bacterial identification by gas chromatography of whole cell fatty acids. Application note 228-241. Palo Alto, CA: Hewlett-Packard.
  27. Pagilla, K. R., Sood, A. & Kim, H.(2002).Gordonia (Nocardia) amarae foaming due to biosurfactant production. Water Sci Technol 46, (1–2). 519–524. [Google Scholar]
  28. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  29. Shen, F. T., Goodfellow, M., Jones, A. L., Chen, Y. P., Arun, A. B., Lai, W. A., Rekha, P. D. & Young, C. C.(2006).Gordonia soli sp. nov., a novel actinomycete isolated from soil. Int J Syst Evol Microbiol 56, 2597–2601.[CrossRef] [Google Scholar]
  30. Shen, F. T., Huang, H. R., Arun, A. B., Lu, H. L., Lin, T. C., Rekha, P. D. & Young, C. C.(2007). Detection of filamentous genus Gordonia in foam samples using genus-specific primers combined with PCR-denaturing gradient gel electrophoresis analysis. Can J Microbiol 53, 768–774.[CrossRef] [Google Scholar]
  31. Soddell, J. A., Stainsby, F. M., Eales, K. L., Seviour, R. J. & Goodfellow, M.(2006).Gordonia defluvii sp. nov., an actinomycete isolated from activated sludge foam. Int J Syst Evol Microbiol 56, 2265–2269.[CrossRef] [Google Scholar]
  32. Stackebrandt, E., Smida, J. & Collins, M. D.(1988). Evidence of phylogenetic heterogeneity within the genus Rhodococcus: revival of the genus Gordona (Tsukamura). J Gen Appl Microbiol 34, 341–348.[CrossRef] [Google Scholar]
  33. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L.(1997). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef] [Google Scholar]
  34. Takeuchi, M. & Hatano, K.(1998).Gordonia rhizosphera sp. nov. isolated from the mangrove rhizosphere. Int J Syst Bacteriol 48, 907–912.[CrossRef] [Google Scholar]
  35. Thompson, K. T., Crocker, F. H. & Fredrickson, H. L.(2005). Mineralization of the cyclic nitramine explosive hexahydro-1,3,5-trinitro-1,3,5-triazine by Gordonia and Williamsia spp. Appl Environ Microbiol 71, 8265–8272.[CrossRef] [Google Scholar]
  36. Tsukamura, M.(1971). Proposal of a new genus, Gordona, for slightly acid-fast organisms occurring in sputa of patients with pulmonary disease and in soil. J Gen Microbiol 68, 15–26.[CrossRef] [Google Scholar]
  37. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  38. Xue, Y., Sun, X., Zhou, P., Liu, R., Liang, F. & Ma, Y.(2003).Gordonia paraffinivorans sp. nov., a hydrocarbon-degrading actinomycete isolated from an oil-producing well. Int J Syst Evol Microbiol 53, 1643–1646.[CrossRef] [Google Scholar]

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[PDF file of Supplementary Tables S1 and S2](46 KB)


Comparison of riboprints of Chol-3 (top) and DSM 44576 (bottom) generated with II.


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