1887

Abstract

The taxonomic position of the cholesterol-degrading strain Chol-3, isolated from a sewage sludge sample, was clarified using a polyphasic taxonomic approach. Phylogenetic analysis of its 16S rRNA gene sequence, whole-cell fatty acid profile and mycolic acid composition revealed that this isolate is a member of the genus with the species , and being the nearest phylogenetic neighbours. The results of DNA–DNA hybridization against its phylogenetically closest neighbours as well as the results of physiological and biochemical tests allowed genotypic and phenotypic differentiation of strain Chol-3 from the other species with validly published names. Strain Chol-3 therefore merits recognition as a member of a novel species within the genus , for which the name sp. nov. is proposed. The type strain is Chol-3 (=CECT 7408 =DSM 45229).

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2009-05-01
2019-10-21
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Comparison of riboprints of Chol-3 (top) and DSM 44576 (bottom) generated with II.

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