Two strains, CN44 and CN47, isolated from marine sediment of the East China Sea, were characterized by using a polyphasic approach. The isolates were Gram-negative, strictly aerobic, non-spore-forming rods. The chemotaxonomic characteristics of these isolates included the presence of C 7, C, iso-C 2-OH and/or C 7 and C 3-OH as the major cellular fatty acids and Q-8 as the predominant ubiquinone. The DNA G+C contents of strains CN44 and CN47 were 62.5 and 56.3 mol%, respectively. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain CN44 was related to members of the genus . The most closely related described organism was the type strain of (95.3 % sequence similarity). Strain CN47 showed the highest sequence similarity to the type strain of (97.8 %) and <97 % similarity to other type strains of described species. The level of DNA–DNA relatedness between strain CN47 and DSM 7027 was 46 %. On the basis of phenotypic and genotypic properties, strains CN44 and CN47 represent two novel species within the genus , for which the names sp. nov. (type strain, CN44 =CGMCC 1.7286 =JCM 15523) and sp. nov. (type strain, CN47 =CGMCC 1.7287 =JCM 15524) are proposed.


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vol. , part 5, pp. 1173 – 1178

Fatty acid composition of strains CN44 and CN47 [ PDF] (95 KB)


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