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Abstract

A Gram-stain-negative, strictly aerobic bacterium, designated strain N7, was isolated from a rice paddy in South Korea. Cells of strain N7 were non-motile, non-spore-forming rods. Growth was observed at 15–35 °C (optimum of 25–30 °C) and between pH 6.0 and 8.0 (optimum of pH 6.5–7.5). The predominant isoprenoid quinone was menaquinone-7. The major cellular fatty acids of strain N7 were summed feature 3 (comprising C 7 and/or iso-C 2-OH), iso-C, anteiso-C, C and iso-C. The G+C content of the genomic DNA was 37.7 mol%. Comparative 16S rRNA gene sequence analyses showed that strain N7 formed a distinct phyletic line within the genus . Phylogenetic distances from strains of other species with validly published names were greater than 5.0 % (i.e. <95.0 % 16S rRNA gene sequence similarities). On the basis of phenotypic and molecular data, it is clear that strain N7 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is N7 (=KACC 12821 =DSM 19973).

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2009-10-01
2021-10-27
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vol. , part 10, pp. 2491 - 2495

Transmission electron micrograph showing the general morphology of a negatively stained cell of strain N7 after growth for 2 days at 30 °C on R2A agar. Bar, 700 nm.



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