A novel, Gram-positive bacterial strain, F12, was isolated from the abdominal epidermis of a sea cucumber, , collected from seawater off the coast of Japan. According to 16S rRNA gene sequence analysis, this strain represents a novel, deep-rooting lineage within the class and clusters with uncultured bacteria and . Compared to species with validly published names, the highest 16S rRNA gene sequence similarity (89.8 %) was to DSM 10331. Phylogenetic analyses showed that strain F12 represents a distinct phylogenetic lineage related closely to the genus . Strain F12 contained MK-9(H) as the major menaquinone, whilst 17 : 0, 17 : 18, 15 : 0 and 16 : 0 were the major cellular fatty acids. The cell-wall peptidoglycan of strain F12 was composed of -diaminopimelic acid as the diagnostic diamino acid, alanine and glutamic acid (1 : 2 : 1). The cell-wall sugars detected were rhamnose, mannose, arabinose, galactose and xylose. The G+C content of the DNA was 74.4 mol%. From the taxonomic data obtained in this study, the name gen. nov., sp. nov. is proposed for the isolate, with type strain F12 (=NBRC 102561=DSM 19957). The name fam. nov. is also proposed for the distinct phyletic line represented by the genus .


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vol. , part 4, pp. 869 – 873

General morphology of cells of F12 viewed by transmission electron microscopy after negative staining. The cells were grown on MA medium for 3 weeks at 30 °C. Bar, 500 nm.


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