1887

Abstract

The taxonomic status of a bacterium isolated from a patient with bronchial carcinoma was established using a polyphasic taxonomic approach. The strain had morphological and chemotaxonomic characteristics consistent with those of members of the genus . The generic assignment was confirmed by comparative analysis of the 16S rRNA gene sequence, which showed that the strain constituted a distinct phyletic line, displaying 93.5–96.9 % sequence similarity with respect to members of the genus . The isolate was distinguished from the type strains of recognized members of the genus by means of various biochemical tests. The genotypic and phenotypic data obtained demonstrated that strain IMMIB L-1070 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is IMMIB L-1070 (=DSM 45226=CCUG 56401).

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2009-05-01
2019-10-14
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References

  1. Becker, B., Lechevalier, M. P., Gordon, R. E. & Lechevalier, H. A. ( 1964; ). Rapid differentiation between Nocardia and Streptomyces by paper chromatography of whole-cell hydrolysates. Appl Microbiol 12, 421–423.
    [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E. ( 1977; ). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef]
    [Google Scholar]
  3. Embley, T. M. ( 1992; ). The family Pseudonocardiaceae. In The Prokaryotes, vol. 1, pp. 996–1027. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. Berlin: Springer.
  4. Felsenstein, J. ( 1981; ). Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17, 368–376.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  7. Goodfellow, M., Lacey, J., Athalye, M., Embley, T. M. & Bowen, T. ( 1989; ). Saccharopolyspora gregorii and Saccharopolyspora hordei: two new actinomycete species from fodder. J Gen Microbiol 135, 2125–2139.
    [Google Scholar]
  8. Gordon, R. E. ( 1966; ). Some criteria for the recognition of Nocardia madurae (Vincent) Blanchard. J Gen Microbiol 45, 355–364.[CrossRef]
    [Google Scholar]
  9. Gordon, R. E. ( 1967; ). The taxonomy of soil bacteria. In The Ecology of Soil Bacteria, pp. 293–321. Edited by T. R. G. Gray & B. Parkinson. Liverpool: Liverpool University Press.
  10. Gordon, R. E. & Mihm, J. M. ( 1957; ). A comparative study of some strains received as nocardiae. J Bacteriol 73, 15–27.
    [Google Scholar]
  11. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  12. Korn-Wendisch, F., Kempf, A., Grund, E., Kroppenstedt, R. M. & Kutzner, H. J. ( 1989; ). Transfer of Faenia rectivirgula Kurup and Agre 1983 to the genus Saccharopolyspora Lacey and Goodfellow 1975, elevation of Saccharopolyspora hirsuta subsp. taberi Labeda 1987 to species level, and emended description of the genus Saccharopolyspora. Int J Syst Bacteriol 39, 430–441.[CrossRef]
    [Google Scholar]
  13. Labeda, D. P. ( 1987; ). Transfer of the type strain of Streptomyces erthyraeus (Waksman 1923) Waksman and Henrici 1948 to the genus Saccharopolyspora Lacey and Goodfellow 1975 as Saccharopolyspora erythraea sp. nov., and designation of a neotype strain for Streptomyces erythraeus. Int J Syst Bacteriol 37, 19–22.[CrossRef]
    [Google Scholar]
  14. Lacey, J. & Goodfellow, M. ( 1975; ). A novel actinomycete from sugar-cane bagasse: Saccharopolyspora hirsuta gen. et sp. nov. J Gen Microbiol 88, 75–85.[CrossRef]
    [Google Scholar]
  15. Lechevalier, M. P. ( 1968; ). Identification of aerobic actinomycetes of clinical importance. J Lab Clin Med 71, 934–944.
    [Google Scholar]
  16. Lechevalier, M. P. & Lechevalier, H. A. ( 1970; ). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef]
    [Google Scholar]
  17. Lechevalier, M. P., De Bièvre, C. & Lechevalier, H. A. ( 1977; ). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef]
    [Google Scholar]
  18. Lu, Z., Liu, Z., Wang, L., Qi, W. & Goodfellow, M. ( 2001; ). Saccharopolyspora flava sp. nov. and Saccharopolyspora thermophila sp. nov., novel actinomycetes from soil. Int J Syst Evol Microbiol 51, 319–325.
    [Google Scholar]
  19. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  20. Mertz, F. P. & Yao, R. C. ( 1990; ). Saccharopolyspora spinosa sp. nov. isolated from soil collected in a sugar mill rum still. Int J Syst Bacteriol 40, 34–39.[CrossRef]
    [Google Scholar]
  21. Minnikin, D. E., Hutchinson, I. G., Caldicott, A. B. & Goodfellow, M. ( 1980; ). Thin-layer chromatography of methanolysates of mycolic acid-containing bacteria. J Chromatogr 188, 221–233.[CrossRef]
    [Google Scholar]
  22. Pimentel-Elardo, S. M., Tiro, L. P., Grozdanov, L. & Hentschel, U. ( 2008; ). Saccharopolyspora cebuensis sp. nov., a novel actinomycete isolated from a Philippine sponge (Porifera). Int J Syst Evol Microbiol 58, 628–632.[CrossRef]
    [Google Scholar]
  23. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsiaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef]
    [Google Scholar]
  24. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  25. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  26. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  27. Yassin, A. F. & Hupfer, H. ( 2006; ). Williamsia deligens sp. nov., isolated from human blood. Int J Syst Evol Microbiol 56, 193–197.[CrossRef]
    [Google Scholar]
  28. Yassin, A. F., Haggenei, B., Budzikiewicz, H. & Schaal, K. P. ( 1993; ). Fatty acid and polar lipid composition of the genus Amycolatopsis: application of fast atom bombardment-mass spectrometry to structure analysis of underivatized phospholipids. Int J Syst Bacteriol 43, 414–420.[CrossRef]
    [Google Scholar]
  29. Yassin, A. F., Rainey, F. A., Brzezinka, H., Burghardt, J., Lee, H. L. & Schaal, K. P. ( 1995; ). Tsukamurella inchonensis sp. nov. Int J Syst Bacteriol 45, 522–527.[CrossRef]
    [Google Scholar]
  30. Yassin, A. F., Chen, W.-M., Hupfer, H., Siering, C., Kroppenstedt, R. M., Arun, A. B., Lai, W.-A., Shen, F.-T., Rekha, P. D. & Young, C. C. ( 2007; ). Lysobacter defluvii sp. nov., isolated from municipal solid waste. Int J Syst Evol Microbiol 57, 1131–1136.[CrossRef]
    [Google Scholar]
  31. Yuan, L.-J., Zhang, Y.-Q., Guan, Y., Wei, Y.-Z., Li, Q.-P., Yu, L.-Y., Li, W.-J. & Zhang, Y.-Q. ( 2008; ). Saccharopolyspora antimicrobica sp. nov., an actinomycete from soil. Int J Syst Evol Microbiol 58, 1180–1185.[CrossRef]
    [Google Scholar]
  32. Zhang, J., Wu, D., Zhang, J., Liu, Z. & Song, F. ( 2008; ). Saccharopolyspora shandongensis sp. nov., isolated from wheat-field soil. Int J Syst Evol Microbiol 58, 1094–1099.[CrossRef]
    [Google Scholar]
  33. Zhou, Z. H., Liu, Z. H., Qian, Y. D., Kim, S. B. & Goodfellow, M. ( 1998; ). Saccharopolyspora spinosporotrichia sp. nov., a novel actinomycete from soil. Int J Syst Bacteriol 48, 53–58.[CrossRef]
    [Google Scholar]
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