1887

Abstract

A novel marine actinomycete, designated SCSIO 00652, was isolated from a marine sediment collected from the northern South China Sea at a depth of 3865 m. The strain formed branched substrate mycelia and no fragmentation was found. Abundant aerial mycelia differentiated into long spore chains and the spores had a wrinkled surface. Growth occurred on ISP medium 2 with 0–5 % (w/v) NaCl and at 10–55 °C. The whole-cell hydrolysate contained -diaminopimelic acid and glucose as the whole-cell sugar. search results based on an almost-complete 16S rRNA gene sequence showed the novel strain had the highest similarity (96.5 %) with VKM Ac-942. The phylogenetic tree of the family indicated that strain SCSIO 00652 formed a distinct lineage at the deepest branch with a high bootstrap value. Additionally, the profiles of menaquinones, phospholipids and fatty acids showed there were marked differences between strain SCSIO 00652 and the recognized genera of the family . Based on the polyphasic data, a new genus, gen. nov., is proposed within the family with the type species sp. nov. The type strain of the type species is SCSIO 00652 (=DSM 45154=CCTCC AA 208041).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.005231-0
2009-05-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/5/948.html?itemId=/content/journal/ijsem/10.1099/ijs.0.005231-0&mimeType=html&fmt=ahah

References

  1. Cai, M., Zhi, X. Y., Tang, S. K., Zhang, Y. Q., Xu, L. H. & Li, W. J.(2008).Streptomonospora halophila sp. nov., a halophilic actinomycete isolated from a hypersaline soil. Int J Syst Evol Microbiol 58, 1556–1560.[CrossRef] [Google Scholar]
  2. Collins, M. D. & Jones, D.(1980). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. J Appl Bacteriol 48, 459–470.[CrossRef] [Google Scholar]
  3. Cui, X. L., Mao, P. H., Zeng, M., Li, W. J., Zhang, L. P., Xu, L. H. & Jiang, C. L.(2001).Streptomonospora salina gen. nov., sp. nov., a new member of the family Nocardiopsaceae. Int J Syst Evol Microbiol 51, 357–363. [Google Scholar]
  4. Dixit, V. S. & Pant, A.(2000). Hydrocarbon degradation and protease production by Nocardiopsis sp. NCIM 5124. Lett Appl Microbiol 30, 67–69.[CrossRef] [Google Scholar]
  5. Evtushenko, L. I., Taran, V. V., Akimov, V. N., Kroppenstedt, R. M., Tiedje, J. M. & Stackebrandt, E.(2000).Nocardiopsis tropica sp. nov., nov. rev., Nocardiopsis trehalosi sp. nov., nom. rev. and Nocardiopsis dassonvillei subsp. albirubida subsp. nov., comb. nov. Int J Syst Evol Microbiol 50, 73–81.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. Gonzalez, C., Gutierrez, C. & Ramirez, C.(1978).Halobacterium vallismortis sp. nov., an amylolytic and carbohydrate-metabolizing, extremely halophilic bacterium. Can J Microbiol 24, 710–715.[CrossRef] [Google Scholar]
  8. Groth, I., Rodriguez, C., Schútze, B., Schmitz, P., Leistner, E. & Goodfellow, M.(2004). Five novel Kitasatospora species from soil: Kitasatospora arboriphila sp. nov., K. gansuensis sp. nov., K. nipponensis sp. nov., K. paranensis sp. nov. and K. terrestris sp. nov. Int J Syst Evol Microbiol 54, 2121–2129.[CrossRef] [Google Scholar]
  9. Hasegawa, T., Takizaea, M. & Tanida, S.(1983). A rapid analysis for chemical grouping aerobic actinomycetes. J Gen Appl Microbiol 29, 319–322.[CrossRef] [Google Scholar]
  10. Kelly, K. L.(1964).Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC: US Government Printing Office.
  11. Kimura, M.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequence. J Mol Evol 16, 111–120.[CrossRef] [Google Scholar]
  12. Kimura, M.(1983).The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  13. Kroppenstedt, R. M.(1982). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. J Liq Chromatogr 5, 2359–2367.[CrossRef] [Google Scholar]
  14. Kroppenstedt, R. M. & Evtushenko, L. I.(2006). The family Nocardiopsaceae. In The Prokaryotes: A Handbook on the Biology of Bacteria, 3rd edn, vol. 3, pp. 754–795. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer-Verlag.
  15. Kroppenstedt, R. M., Korn-Wendisch, F., Fowler, V. J. & Stackebrandt, E.(1981). Biochemical and molecular genetic evidence for transfer of Actinoplanes armeniacus into the family Streptomycetaceae. Zentralbl Bakt Hyg Abt Orig C 2, 254–262. [Google Scholar]
  16. Li, W. J., Xu, P., Schumann, P., Zhang, Y. Q., Pukall, R., Xu, L. H., Stackebrandt, E. & Jiang, C. L.(2007).Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China) and emended description of the genus Georgenia. Int J Syst Evol Microbiol 57, 1424–1428.[CrossRef] [Google Scholar]
  17. Mesbah, M., Premachandran, U. & Whitman, W. B.(1989). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef] [Google Scholar]
  18. Meyer, J.(1976).Nocardiopsis, a new genus of the order Actinomycetales. Int J Syst Bacteriol 26, 487–493.[CrossRef] [Google Scholar]
  19. Minnikin, D. E., Collins, M. D. & Goodfellow, M.(1979). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. J Appl Bacteriol 47, 87–95.[CrossRef] [Google Scholar]
  20. Minnikin, D. E., O'Donnell, A. G., Goodfellow, M., Alderson, G., Athalye, M., Schaal, A. & Parlett, J. H.(1984). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2, 233–241.[CrossRef] [Google Scholar]
  21. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E.(1996). The genus Norcardiopsis represents a phylogenetically coherent taxon and a distinct actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef] [Google Scholar]
  22. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  23. Sasser, M.(1990). Identification of bacteria by gas chromatography of cellular fatty acids. USFCC Newsl 20, 16 [Google Scholar]
  24. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  25. Staneck, J. L. & Roberts, G. D.(1974). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231. [Google Scholar]
  26. Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef] [Google Scholar]
  27. Tang, S.-K., Tian, X.-P., Zhi, X.-Y., Cai, M., Wu, J.-Y., Yang, L.-L., Xu, L.-H. & Li, W.-J.(2008).Haloactinospora alba gen. nov., sp. nov., a novel halophilic filamentous actinomycete of the family Nocardiopsaceae. Int J Syst Evol Microbiol 58, 2075–2080.[CrossRef] [Google Scholar]
  28. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  29. Vickers, J. C., Williams, S. T. & Ross, G. W.(1984). A taxonomic approach to selective isolation of streptomycetes from soil. In Biological, Biochemical and Biomedical Aspects of Actinomycetes, pp. 553–551. Edited by L. Ortiz-Ortiz, L. F. Bojalil & V. Yakole. London: Academic Press.
  30. Waksman, S. A.(1961).The Actinomycetes, vol. II. Classification, Identification and Description of Genera and Species. Baltimore: Williams & Wilkins.
  31. Yang, L. L., Tang, S. K., Zhang, Y. Q., Zhi, X. Y., Wang, D., Xu, L. H. & Li, W. J.(2008).Thermobifida halotolerans sp. nov., isolated from a salt mine sample, and emended description of the genus Thermobifida. Int J Syst Evol Microbiol 58, 1821–1825.[CrossRef] [Google Scholar]
  32. Zhang, Z., Wang, Y. & Ruan, J.(1998). Reclassification of Thermomonospora and Microtetraspora. Int J Syst Bacteriol 48, 411–422.[CrossRef] [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.005231-0
Loading
/content/journal/ijsem/10.1099/ijs.0.005231-0
Loading

Data & Media loading...

Supplements

vol. , part 5, pp. 948 - 952

. Patterns of selected 16S rRNA gene signature nucleotides detected among the various genera of the family . [ PDF] 58 KB



PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error