A Gram-negative, non-spore-forming, non-motile bacterium, strain A8-7, was isolated from fresh water of a slightly alkaline lake, Longhu Lake, in Daqing, north-east China, and its taxonomic position was studied by using a polyphasic approach. Strain A8-7 was aerobic, heterotrophic and positive for catalase and oxidase. It grew at 20–37 °C (optimum 30 °C) and pH 5.5–10.5 (optimum pH 7.5) and in the presence of 0–3 % (w/v) NaCl. It formed pink-pigmented, smooth and circular colonies, 1–2 mm in diameter, on R3A-V agar plates after incubation at 30 °C for 3 days. Cells of strain A8-7 were rods, 0.2–0.4 μm wide and 1.6–4.0 μm long. The major fatty acids (>10 %) were iso-C (40.3 %) and summed feature 3 (C 7 and/or iso-C 2-OH; 12.1 %). The menaquinone was MK-7. The DNA G+C content was 43 mol% ( ). Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain A8-7 was phylogenetically related to members of the genus , with sequence similarities of 92.6–95.2 %, the highest sequence similarity being to the sequence from IMSNU 14012. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain A8-7 was considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is A8-7 (=CGMCC 1.7030 =NBRC 104237).


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vol. , part 7, pp. 1759 - 1763

Maximum-parsimony and maximum-likelihood trees based on 16S rRNA gene sequences.

Cellular fatty acid contents of strain A8-7 and the type strains of other species.

[PDF file of Supplementary Fig. S1 and Table S1](138 KB)


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