A novel, xylanolytic, anaerobic, moderately thermophilic bacterium, strain PML14T, was isolated from the sludge of a thermophilic anaerobic digester treating municipal solid waste and sewage in Beijing, China. The strain was a Gram-positive, spore-forming and motile rod. Growth of the novel strain was observed at 25–67 °C (optimum 60 °C) and pH 5.8–9.3 (optimum pH 8.5). Strain PML14T grew on a number of carbohydrates, including xylan, xylose, glucose and cellobiose, and a variety of proteinaceous compounds, including peptone, tryptone, Casamino acids, yeast extract, beef extract, casein hydrolysate, l-cysteine, l-serine, l-lysine, l-glycine, l-threonine, l-methionine and pyruvate. The fermentation products from glucose included acetate, ethanol, butyrate, hydrogen and carbon dioxide. Propionate was produced from xylan in addition to other compounds. Fe(III), 9,10-anthraquinone 2,6-disulfonate and thiosulfate were reduced with peptone as the electron donor. NH3 was produced. Indole was not produced. Gelatin was not hydrolysed. The DNA G+C content of strain PML14T was 36.2±0.8 mol% (Tm). Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PML14T was related to the members of cluster XII of the clostridia, most closely to Tepidimicrobium ferriphilum SB91T with 93.8 % 16S rRNA gene sequence similarity. On the basis of polyphasic evidence from this study, it is suggested that strain PML14T (=CGMCC 1.5080T=JCM 15035T) represents a novel species of the genus Tepidimicrobium, for which the name Tepidimicrobiumxylanilyticum sp. nov., is proposed. An emended description of the genus Tepidimicrobium is also provided.
Barrow, G. I. & Feltham, R. K. A.(1993).Cowan and Steel's Manual for the Identification of Medical Bacteria, 3rd edn. Cambridge: Cambridge University Press.
Chen, S. & Dong, X.(2004).Acetanaerobacterium elongatum gen. nov., sp. nov., from paper mill waste water. Int J Syst Evol Microbiol54, 2257–2262.[CrossRef][Google Scholar]
Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. E.(1994). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combination. Int J Syst Bacteriol44, 812–826.[CrossRef][Google Scholar]
Das, D. & Veziroglu, T. N.(2001). Hydrogen production by biological processes: a survey of literature. Int J Hydrogen Energy26, 13–28.[CrossRef][Google Scholar]
Holdeman, L. V., Cato, E. P. & Moore, W. E. C.(1977).Anaerobe Laboratory Manual, 4th edn. Blacksburg, VA: Virginia Polytechnic Institute and State University.
Hungate, R. E.(1969). A roll tube method for cultivation of strict anaerobes. Methods Microbiol3B, 117–132.
[Google Scholar]
Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol3, 208–218.[CrossRef][Google Scholar]
Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol5, 109–118.[CrossRef][Google Scholar]
Nandi, R. & Sengupta, S.(1998). Microbial production of hydrogen: an overview. Crit Rev Microbiol24, 61–84.[CrossRef][Google Scholar]
Niu, L., Song, L. & Dong, X.(2008).Proteiniborus ethanoligenes gen. nov., sp. nov., an anaerobic protein-utilizing bacterium. Int J Syst Evol Microbiol58, 12–16.[CrossRef][Google Scholar]
Schnürer, A., Schink, B. & Svensson, B. H.(1996).Clostridium ultunense sp. nov., a mesophilic bacterium oxidizing acetate in syntrophic association with a hydrogentrophic methanogenic bacterium. Int J Syst Bacteriol46, 1145–1152.[CrossRef][Google Scholar]
Slobodkin, A. I., Tourova, T. P., Kostrikina, N. A., Lysenko, A. M., German, K. E., Bonch-Osmolovskaya, E. A. & Birkeland, N.-K.(2006).Tepidimicrobium ferriphilum gen. nov., sp. nov., a novel moderately thermophilic, Fe(III)-reducing bacterium of the order Clostridiales. Int J Syst Evol Microbiol56, 369–372.[CrossRef][Google Scholar]
Zehnder, A. J. B.(1978). Ecology of methane formation. In Water Pollution Microbiology, pp. 349–376. Edited by R. Mitchell. New York: Wiley.