A mesophilic, aerobic, facultatively chemolithoautotrophic bacterium, designated strain EPR70, was isolated from hydrothermal fluids from diffuse-flow vents on the East Pacific Rise at  ° 50′ N 10 ° 17′ W. Cells were Gram-negative rods, approximately 0.8–1.0 μm long and 0.3–0.5 μm wide. Strain EPR70 grew at 20–40 °C (optimum 30–35 °C), 1–25 % NaCl (optimum 2.5 %) and pH 5.0–7.5 (optimum pH 5.5). The shortest generation time observed for strain EPR70 was 42 min. Growth occurred under aerobic chemolithoautotrophic conditions in the presence of thiosulfate and CO. Strain EPR70 grew heterotrophically with acetate or n-alkanes as sole carbon and energy sources, and in complex artificial seawater medium. Nitrate was not used as an electron acceptor. The G+C content of the genomic DNA was 64 mol%. Phylogenetic analysis of the 16S rRNA gene indicated that this organism is a member of the class , with E1L3A as its closest relative (94 % sequence similarity). On the basis of phylogenetic analyses based on 16S rRNA, and genes and physiological analysis, it is proposed that the organism represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is EPR70 (=DSM 21483 =JCM 15514).


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vol. , part 6, pp. 1497 - 1503

Generation time of sp. nov. EPR70 under different growth conditions.

Neighbour-joining phylogenetic analysis of the large subunit of the enzyme RubisCO form I from sp. nov. EPR70 , sp. strains EPR71 and EPR72 and E1L3A .

Neighbour-joining phylogenetic analysis of alkane hydroxylase (AlkB) from sp. nov. EPR70 , sp. strain EPR71 and E1L3A .

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