1887

Abstract

A novel strictly anaerobic, endospore-forming, rod-shaped, Gram-positive bacterium, designated strain SGB2, was isolated from a mixed culture from a pond sediment during screening for sulfate-reducing bacteria capable of degrading cyanophycin (CGP). In this study, the taxonomic characterization of this mesophilic, proteolytic isolate and the role which it, and its phylogenetic relatives, may play in peptide degradation and in the sulfur cycle are reported. Strain SGB2 was a commensal strain, utilizing CGP degradation products produced by other micro-organisms. Cells were motile until sporulation, forming oval, terminal spores that swell the cells. It showed optimum growth at 34 °C, pH 6.6 and in the absence of NaCl. Strain SGB2 utilized proteinaceous compounds such as peptone, Casamino acids, gelatin and trypticase soy, in addition to several amino acids and pyruvate. Utilization of many of these compounds was enhanced in the presence of thiosulfate. The isolate was unable to use any of the carbohydrates or alcohols investigated or CGP as carbon and energy sources. Thiosulfate and elemental sulfur were used as terminal electron acceptors. Phylogenetic analysis revealed that strain SGB2 belongs to the low-G+C-containing group. It exhibited 99 % 16S rRNA gene sequence similarity to its closest relatives Lup21 and DSM 6970. DNA–DNA hybridization values with these two strains were 39.4 and 42.1 %, respectively. Based on phenotypic, genotypic and phylogenetic characteristics, we conclude that the isolate represents a novel species of the genus , sp. nov. The type strain is SGB2 (=DSM 18982 =ATCC BAA-1538).

Keyword(s): CGP, cyanophycin
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2009-07-01
2019-10-20
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References

  1. Campbell, L. L. & Postgate, J. R. ( 1965; ). Classification of the spore-forming sulfate-reducing bacteria. Bacteriol Rev 29, 359–363.
    [Google Scholar]
  2. Cato, E. P., George, W. L. & Finegold, S. M. ( 1986; ). Genus Clostridium Prazmowski 1880, 23AL. In Bergey's Manual of Systematic Bacteriology, vol. 2, pp. 1141–1200. Edited by P. H. A. Sneath, N. S. Mair, M. E. Sharpe & J. G. Holt. Baltimore: Williams & Wilkins.
  3. Collins, M. D., Lawson, P. A., Willems, A., Cordoba, J. J., Fernandez-Garayzabal, J., Garcia, P., Cai, J., Hippe, H. & Farrow, J. A. E. ( 1994; ). The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44, 812–826.[CrossRef]
    [Google Scholar]
  4. Elsden, S. R. & Hilton, M. G. ( 1979; ). Amino acid utilization patterns in clostridial taxonomy. Arch Microbiol 123, 137–141.[CrossRef]
    [Google Scholar]
  5. Escoffier, S., Ollivier, B., Le Mer, J., Garcin, J. & Roger, P. A. ( 1998; ). Evidence and quantification of thiosulfate-reducers unable to reduce sulfate in rice-field soils. Eur J Soil Biol 34, 69–74.[CrossRef]
    [Google Scholar]
  6. Escoffier, S., Cayol, J.-L., Ollivier, B., Patel, B. K. C., Fardeau, M.-L., Thomas, P. & Roger, P. A. ( 2001; ). Identification of thiosulfate- and sulfur-reducing bacteria unable to reduce sulfate in ricefield soils. Eur J Soil Biol 37, 145–156.[CrossRef]
    [Google Scholar]
  7. Hernández-Eugenio, G., Fardeau, M.-L., Cayol, J.-L., Patel, B. K. C., Thomas, P., Macarie, H., Garcia, J.-L. & Ollivier, B. ( 2002; ). Clostridium thiosulfatireducens sp. nov., a proteolytic, thiosulfate- and sulfur-reducing bacterium isolated from an upflow anaerobic sludge blanket (UASB) reactor. Int J Syst Evol Microbiol 52, 1461–1468.[CrossRef]
    [Google Scholar]
  8. Hippe, H., Andreesen, J. R. & Gottschalk, G. ( 1992; ). The genus Clostridium – nonmedical. In The Prokaryotes, 2nd edn, vol. 2, pp. 1800–1866. Edited by A. Balows, H. G. Trüper, M. Dworkin, W. Harder & K. H. Schleifer. New York: Springer.
  9. Hollaus, F. & Sleytr, U. ( 1972; ). On the taxonomy and fine structure of some hyperthermophilic saccharolytic clostridia. Arch Microbiol 86, 129–146.
    [Google Scholar]
  10. Lee, Y.-E., Jain, M. K., Lee, C., Lowe, S. E. & Zeikus, J. G. ( 1993; ). Taxonomic distinction of saccharolytic thermophilic anaerobes: description of Thermoanaerobacterium xylanolyticum gen. nov., sp. nov., and Thermoanaerobacterium saccharolyticum gen. nov., sp. nov.; reclassification of Thermoanaerobium brockii, Clostridium thermosulfurogenes, and Clostridium thermohydrosulfuricum E100-69 as Thermoanaerobacter brockii comb. nov., Thermoanaerobacterium thermosulfurigenes comb. nov., and Thermoanaerobacter thermohydrosulfuricus comb. nov., respectively; and transfer of Clostridium thermohydrosulfuricum 39E to Thermoanaerobacter ethanolicus. Int J Syst Bacteriol 43, 41–51.[CrossRef]
    [Google Scholar]
  11. Mechichi, T., Fardeau, M.-L., Labat, M., Garcia, J.-L., Verhé, F. & Patel, B. K. C. ( 2000; ). Clostridium peptidivorans sp. nov., a peptide-fermenting bacterium from an olive mill wastewater treatment digester. Int J Syst Evol Microbiol 50, 1259–1264.[CrossRef]
    [Google Scholar]
  12. Obst, M., Krug, A., Luftmann, H. & Steinbüchel, A. ( 2005; ). Degradation of cyanophycin by Sedimentibacter hongkongensis strain KI and Citrobacter amalonaticus strain G isolated from an anaerobic bacterial consortium. Appl Environ Microbiol 71, 3642–3652.[CrossRef]
    [Google Scholar]
  13. Purushothaman, D., Balasundaram, C. S. & Gunasekaran, S. ( 1981; ). Nitrogen fixation in the rhizosphere of rice. Proc Indian Natl Sci Acad B (Biol Sci) 47, 242–247.
    [Google Scholar]
  14. Sallam, A. & Steinbüchel, A. ( 2008; ). Anaerobic and aerobic degradation of cyanophycin by the denitrifying bacterium Pseudomonas alcaligenes strain DIP1 and the role of three other coisolates in a mixed bacterial consortium. Appl Environ Microbiol 74, 3434–3443.[CrossRef]
    [Google Scholar]
  15. Schink, B. & Zeikus, J. G. ( 1983; ). Clostridium thermosulfurogenes sp. nov., a new thermophile that produces elemental sulphur from thiosulphate. J Gen Microbiol 129, 1149–1158.
    [Google Scholar]
  16. Simon, R. D. & Weathers, P. ( 1976; ). Determination of the structure of the novel polypeptide containing aspartic acid and arginine which is found in cyanobacteria. Biochim Biophys Acta 420, 165–176.[CrossRef]
    [Google Scholar]
  17. Stackebrandt, E. & Rainey, F. A. ( 1997; ). Phylogenetic relationships. In The Clostridia: Molecular Biology and Pathogenesis, pp. 3–19. Edited by J. I. Rood, B. A. McClane, J. G. Songer & R. W. Titball. New York: Academic Press.
  18. Thabet, O. B. D., Fardeau, M. L., Joulian, C., Thomas, P., Hamdi, M., Garcia, J. L. & Ollivier, B. ( 2004; ). Clostridium tunisiense sp. nov., a new proteolytic, sulfur-reducing bacterium isolated from an olive mill wastewater contaminated by phosphogypse. Anaerobe 10, 185–190.[CrossRef]
    [Google Scholar]
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