1887

Abstract

Two Gram-stain-positive, pigmented, non-motile, non-spore-forming, pleomorphic, rod-shaped bacteria (strains SV45 and SV47), isolated from a permafrost soil collected from the Adventdalen valley, Spitsbergen, northern Norway, have been characterized taxonomically using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two permafrost isolates formed a distinct phyletic line within the suborder of the order . DNA–DNA hybridization analyses indicate that strains SV45 and SV47 are closely related (60–69 % relatedness) and belong to the same species, although they show slightly different colony pigmentation. The closest phylogenetic neighbour was JC2054, with 96 % 16S rRNA gene sequence similarity. Optimum growth of SV45 and SV47 occurred aerobically in the absence of NaCl, but both isolates tolerated up to 2 % NaCl (w/v) in the growth medium. Growth under anaerobic conditions was slow and weak. The peptidoglycan of both isolates was of the A4 type with -ornithine as the diamino acid and serine as a component of the interpeptide bridge with either -aspartate (SV45) or -glutamate (SV47) as the N-terminal amino acid. The major fatty acids present in both isolates were C (3.2–8.6 %), iso-C (5.0–8.9 %), anteiso-C (59.4–61.5 %), anteiso-C (4.1–8.8 %) and anteiso-C (4.4–6.4 %). Isoprenoid quinones were present at exceptionally low levels in both isolates, and only demethylmenaquinone DMK-9(H) could be identified with any degree of confidence. Phylogenetic analysis and differences in physiological and biochemical characteristics between the strains and JC2054 indicate that these isolates belong to a novel species within the genus , for which the name sp. nov. is proposed. The type strain is SV45 (=LMG 24795 =DSM 19970).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.004929-0
2009-04-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/4/649.html?itemId=/content/journal/ijsem/10.1099/ijs.0.004929-0&mimeType=html&fmt=ahah

References

  1. An, D.-S., Im, W.-T., Yang, H.-C., Kang, M. S., Kim, K. K., Jin, L., Kim, M. K. & Lee, S.-T. ( 2005; ). Cellulomonas terrae sp. nov., a cellulolytic and xylanolytic bacterium isolated from soil. Int J Syst Evol Microbiol 55, 1705–1709.[CrossRef]
    [Google Scholar]
  2. Bagnara, C., Toci, R., Gaudin, C. & Belaich, J. P. ( 1985; ). Isolation and characterization of a cellulolytic microorganism, Cellulomonas fermentans sp. nov. Int J Syst Bacteriol 35, 502–507.[CrossRef]
    [Google Scholar]
  3. Cowan, S. T. & Steel, K. J. ( 1993; ). Manual for the Identification of Medical Bacteria, 3rd edn. Edited by G. I. Barrow & R. K. A. Feltham. Cambridge: Cambridge University Press.
  4. Felsenstein, J. ( 2007; ). phylip (phylogeny inference package) version 3.67. Distributed by the author. Department of Genome Sciences, University of Washington, Seattle, USA.
  5. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  6. Hansen, A. A., Herbert, R. A., Mikkelsen, K., Jensen, L. L., Kristoffersen, T., Tiedje, J. M., Lomstein, B. A. & Finster, K. W. ( 2007; ). Viability, diversity and composition of the bacterial community in a high Arctic permafrost soil from Northern Norway. Environ Microbiol 9, 2870–2884.[CrossRef]
    [Google Scholar]
  7. Jones, B. E., Grant, W. D., Duckworth, A. W., Schumann, P., Weiss, N. & Stackebrandt, E. ( 2005; ). Cellulomonas bogoriensis sp. nov., an alkaliphilic cellulomonad. Int J Syst Evol Microbiol 55, 1711–1714.[CrossRef]
    [Google Scholar]
  8. Ludwig, W., Strunk, O., Westram, R., Richter, L., Meier, H., Yadhukumar, Buchner, A., Lai, T., Steppi, S. & other authors ( 2004; ). arb: a software environment for sequence data. Nucleic Acids Res 32, 1363–1371.[CrossRef]
    [Google Scholar]
  9. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  10. Pruesse, E., Quast, C., Knittel, K., Fuchs, B., Ludwig, W., Peplies, J., Glöckner, F. & Silva, O. ( 2007; ). A comprehensive online resource for quality checked and aligned ribosomal RNA sequence data compatible with arb. Nucleic Acids Res 35, 7188–7196.[CrossRef]
    [Google Scholar]
  11. Rivas, R., Trujillo, M. E., Mateos, P. F., Martínez-Molina, E. & Velázquez, E. ( 2004; ). Cellulomonas xylanilytica sp. nov., a cellulolytic and xylanolytic bacterium isolated from a decayed elm tree. Int J Syst Evol Microbiol 54, 533–536.[CrossRef]
    [Google Scholar]
  12. Ronquist, F. & Huelsenbeck, J. P. ( 2003; ). MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics 19, 1572–1574.[CrossRef]
    [Google Scholar]
  13. Schleifer, K. H. & Seidl, P. H. ( 1985; ). Chemical composition and structure of murein. In Chemical Methods in Bacterial Systematics, pp. 201–219. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  14. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  15. Stackebrandt, E., Rainey, F. A. & Ward-Rainey, N. L. ( 1997; ). Proposal for a new hierarchic classification system, Actinobacteria classis nov. Int J Syst Bacteriol 47, 479–491.[CrossRef]
    [Google Scholar]
  16. Stackebrandt, E., Schumann, P. & Prauser, H. ( 2006; ). The family Cellulomonadaceae. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 3, pp. 983–1001. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  17. Staneck, J. L. & Roberts, G. D. ( 1974; ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. Appl Microbiol 28, 226–231.
    [Google Scholar]
  18. Yi, H., Schumann, P. & Chun, J. ( 2007; ). Demequina aestuarii gen. nov., sp. nov., a novel actinomycete of the suborder Micrococcineae, and reclassification of Cellulomonas fermentans Bagnara et al. 1985 as Actinotalea fermentans gen. nov., comb. nov. Int J Syst Evol Microbiol 57, 151–156.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.004929-0
Loading
/content/journal/ijsem/10.1099/ijs.0.004929-0
Loading

Data & Media loading...

Supplements

vol. , part 4, pp. 649 - 653

Phylogenetic position of strains SV45 and SV47 among selected taxa affiliated with the actinobacterial suborder . The tree includes representatives of the type species of all named genera affiliated with the suborder according to the most recent version (May 2008) of the NCBI taxonomy database (Wheeler , 2000). [PDF](271 KB)

Database resources of the National Center for Biotechnology Information. , 10–14.



PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error