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Abstract

Two Gram-stain-positive, pigmented, non-motile, non-spore-forming, pleomorphic, rod-shaped bacteria (strains SV45 and SV47), isolated from a permafrost soil collected from the Adventdalen valley, Spitsbergen, northern Norway, have been characterized taxonomically using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the two permafrost isolates formed a distinct phyletic line within the suborder of the order . DNA–DNA hybridization analyses indicate that strains SV45 and SV47 are closely related (60–69 % relatedness) and belong to the same species, although they show slightly different colony pigmentation. The closest phylogenetic neighbour was JC2054, with 96 % 16S rRNA gene sequence similarity. Optimum growth of SV45 and SV47 occurred aerobically in the absence of NaCl, but both isolates tolerated up to 2 % NaCl (w/v) in the growth medium. Growth under anaerobic conditions was slow and weak. The peptidoglycan of both isolates was of the A4 type with -ornithine as the diamino acid and serine as a component of the interpeptide bridge with either -aspartate (SV45) or -glutamate (SV47) as the N-terminal amino acid. The major fatty acids present in both isolates were C (3.2–8.6 %), iso-C (5.0–8.9 %), anteiso-C (59.4–61.5 %), anteiso-C (4.1–8.8 %) and anteiso-C (4.4–6.4 %). Isoprenoid quinones were present at exceptionally low levels in both isolates, and only demethylmenaquinone DMK-9(H) could be identified with any degree of confidence. Phylogenetic analysis and differences in physiological and biochemical characteristics between the strains and JC2054 indicate that these isolates belong to a novel species within the genus , for which the name sp. nov. is proposed. The type strain is SV45 (=LMG 24795 =DSM 19970).

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2009-04-01
2021-07-23
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vol. , part 4, pp. 649 - 653

Phylogenetic position of strains SV45 and SV47 among selected taxa affiliated with the actinobacterial suborder . The tree includes representatives of the type species of all named genera affiliated with the suborder according to the most recent version (May 2008) of the NCBI taxonomy database (Wheeler , 2000). [PDF](271 KB)

Database resources of the National Center for Biotechnology Information. , 10–14.



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