RT Journal Article SR Electronic(1) A1 Zhang, Lei A1 Wang, Yang A1 Dai, Jun A1 Tang, Yali A1 Yang, Qiao A1 Luo, Xuesong A1 Fang, ChengxiangYR 2009 T1 Bacillus korlensis sp. nov., a moderately halotolerant bacterium isolated from a sand soil sample in China JF International Journal of Systematic and Evolutionary Microbiology, VO 59 IS 7 SP 1787 OP 1792 DO https://doi.org/10.1099/ijs.0.004879-0 PB Microbiology Society, SN 1466-5034, AB A Gram-positive-staining, rod-shaped, motile, spore-forming and moderately halotolerant bacterium, designated ZLC-26T, was isolated from a sand soil sample collected from Xinjiang Province, China, and was characterized by using a polyphasic taxonomic approach. This isolate grew optimally at 30–37 °C and pH 7–8. It grew with 0–8% NaCl (optimum, 0–2 %). Comparative 16S rRNA gene sequence analysis showed that strain ZLC-26T was closely related to members of the genus Bacillus, exhibiting the highest 16S rRNA gene sequence similarities to Bacillus nealsonii DSM 15077T (97.1 %), B. shackletonii LMG 18435T (97.0 %), B. siralis 171544T (97.0 %), B. circulans IAM 12462T (96.7 %) and B. pocheonensis Gsoil 420T (96.7 %). Strain ZLC-26T contained MK-7 as the predominant menaquinone. The diagnostic diamino acid in the cell-wall peptidoglycan was meso-diaminopimelic acid. The major cellular fatty acids were iso-C15 : 0, C16 : 1 ω11c and anteiso-C15 : 0. The DNA G+C content was 38.2 mol%. These chemotaxonomic results supported the affiliation of strain ZLC-26T to the genus Bacillus. However, low DNA–DNA relatedness values and distinguishing phenotypic characteristics allowed genotypic and phenotypic differentiation of strain ZLC-26T from recognized Bacillus species. On the basis of the evidence presented, strain ZLC-26T is considered to represent a novel species of the genus Bacillus, for which the name Bacillus korlensis sp. nov. is proposed. The type strain is ZLC-26T (=CCTCC AB 207172T=NRRL B-51302T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.004879-0