1887

International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 7

sp. nov. and sp. nov., isolated from soil Free

Abstract

Two novel strains isolated from soils, JS12-10 and JS14-6, were characterized using a polyphasic approach to determine their taxonomic positions. These isolates were found to be aerobic, Gram-negative, motile with one polar flagellum, non-spore-forming and rod-shaped. Phenotypic and fatty acid data supported the affiliation of JS12-10 and JS14-6 to the genus . However, chemotaxonomic data and DNA–DNA relatedness values allowed differentiation of these strains from other species with validly published names. Strains JS12-10 and JS14-6 showed the highest 16S rRNA gene sequence similarities with Gsoil 3046 (98.4 %) and XD53 (97.9 %), respectively, and the 16S rRNA gene sequence similarity between them was 97.1 %. DNA–DNA hybridization values between the novel isolates and strains of other recognized species were 29–38 %. Therefore, strains JS12-10 and JS14-6 represent two novel species of the genus , for which the names sp. nov. (type strain JS12-10 =KACC 12747 =JCM 15423) and sp. nov. (type strain JS14-6 =KACC 12748 =JCM 15424) are proposed.

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Keyword(s): DIG, digoxigenin–11-dUTP , ML, maximum-likelihood , MP, maximum-parsimony and NJ, neighbour-joining
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2009-07-01
2019-10-19
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Dyella soli sp. nov. and Dyella terrae sp. nov., isolated from soil
Int J Syst Evol Microbiol 59, 1685 (2009); https://doi.org/10.1099/ijs.0.004838-0
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vol. , part 7, pp. 1685 - 1690

Maximum-likelihood and maximum-parsimony trees based on almost-complete 16S rRNA gene sequences showing the phylogenetic position of strains JS12-10 and JS14-6 . [PDF](28 KB)

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