1887

Abstract

Two yellow-coloured bacterial strains, designated JS13-10 and JS16-4, were isolated from soil from Jeju Island, Republic of Korea. On the basis of 16S rRNA gene sequence analysis, the strains were found to be affiliated with members of the genus . Phenotypically, the novel strains were identified as being different from each other and from recognized species of the genus . DNA–DNA hybridization tests between the two novel strains and closely related reference strains produced DNA relatedness values that were significantly lower (<36 %) than those generally accepted as the highest threshold for the phylogenetic definition of a species. On the basis of their distinct taxonomic characteristics, these strains represent two novel species of the genus , for which the names sp. nov. (type strain JS13-10=KACC 12952=JCM 15440) and sp. nov. (type strain JS16-4=KACC 12954=JCM 15441) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.004804-0
2009-06-01
2020-01-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/6/1267.html?itemId=/content/journal/ijsem/10.1099/ijs.0.004804-0&mimeType=html&fmt=ahah

References

  1. An, D.-S., Im, W.-T., Yoon, M.-H., Lee, S.-T. & Choi, W.-Y. ( 2007; ). Chitinophaga soli sp. nov. and Chitinophaga terrae sp. nov., isolated from soil of a ginseng field in Pocheon province, South Korea. J Microbiol Biotechnol 17, 705–711.
    [Google Scholar]
  2. Breznak, J. A. & Costilow, R. N. ( 1994; ). Physicochemical factors in growth. In Methods for General and Molecular Bacteriology, pp. 137–154. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  3. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  4. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  5. Fujita, T., Okamoto, M., Kosako, Y. & Okuhura, M. ( 1996; ). Flexibacter japonensis sp. nov., a new species that produces a novel inhibitor of human leukocyte elastase isolated from soil. Curr Microbiol 33, 89–93.[CrossRef]
    [Google Scholar]
  6. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A. ( 1996; ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef]
    [Google Scholar]
  7. Kämpfer, P., Young, C. C., Sridhar, K. R., Arun, A. B., Lai, W. A., Shen, F. T. & Rekha, P. D. ( 2006; ). Transfer of [Flexibacter] sancti, [Flexibacter] filiformis, [Flexibacter] japonensis and [Cytophaga] arvensicola to the genus Chitinophaga and description of Chitinophaga skermanii sp. nov. Int J Syst Evol Microbiol 56, 2223–2228.[CrossRef]
    [Google Scholar]
  8. Kim, M. K. & Jung, H.-Y. ( 2007; ). Chitinophaga terrae sp. nov., isolated from soil. Int J Syst Evol Microbiol 57, 1721–1724.[CrossRef]
    [Google Scholar]
  9. Kimura, M. ( 1983; ). The Neutral Theory of Molecular Evolution. Cambridge: Cambridge University Press.
  10. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  11. Kwon, S.-W., Kim, J.-S., Park, I.-C., Yoon, S.-H., Park, D.-H., Lim, C.-K. & Go, S.-J. ( 2003; ). Pseudomonas koreensis sp. nov., Pseudomonas umsongensis sp. nov. and Pseudomonas jinjuensis sp. nov., novel species from farm soils in Korea. Int J Syst Evol Microbiol 53, 21–27.[CrossRef]
    [Google Scholar]
  12. Lee, H.-G., An, D.-S., Im, W.-T., Liu, Q.-M., Na, J.-R., Cho, D. H., Jin, C. W., Lee, S.-T. & Yang, D.-C. ( 2007; ). Chitinophaga ginsengisegetis sp. nov. and Chitinophaga ginsengisoli sp. nov., isolated from soil of a ginseng field in South Korea. Int J Syst Evol Microbiol 57, 1396–1401.[CrossRef]
    [Google Scholar]
  13. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  14. Pankratov, T. A., Kulichevskaya, I. S., Liesack, W. & Dedysh, S. N. ( 2006; ). Isolation of aerobic, gliding, xylanolytic and laminarinolytic bacteria from acidic Sphagnum peatlands and emended description of Chitinophaga arvensicola Kämpfer et al. 2006. Int J Syst Evol Microbiol 56, 2761–2764.[CrossRef]
    [Google Scholar]
  15. Reasoner, D. J. & Geldreich, E. E. ( 1985; ). A new medium for the enumeration and subculture of bacteria from potable water. Appl Environ Microbiol 49, 1–7.
    [Google Scholar]
  16. Reichenbach, H. ( 1989; ). Genus Flexibacter Soriano 1945, 92,AL emend. In Bergey's Manual of Systematic Bacteriology, vol. 3, pp. 2061–2071. Edited by J. T. Staley, M. P. Bryant, N. Pfennig & J. G. Holt. Baltimore: Williams & Wilkins.
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Sangkhobol, V. & Skerman, V. B. D. ( 1981; ). Chitinophaga, a new genus of chitinolytic myxobacteria. Int J Syst Bacteriol 31, 285–293.[CrossRef]
    [Google Scholar]
  19. Seldin, L. & Dubnau, D. ( 1985; ). Deoxyribonucleic acid homology among Bacillus polymyxa, Bacillus macerans, Bacillus azotofixans, and other nitrogen-fixing Bacillus strains. Int J Syst Bacteriol 35, 151–154.[CrossRef]
    [Google Scholar]
  20. Smibert, R. M. & Krieg, N. R. ( 1994; ). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  21. Thompson, J. D., Higgins, D. G. & Gibson, T. J. ( 1994; ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22, 4673–4680.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.004804-0
Loading
/content/journal/ijsem/10.1099/ijs.0.004804-0
Loading

Data & Media loading...

Most cited articles

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error