The taxonomic position of a yellow-pigmented bacterial strain, designated DS20, isolated from a hexachlorocyclohexane dump site at Lucknow, India was determined based on a polyphasic taxonomic characterization. A neighbour-joining tree based on 16S rRNA gene sequences showed that strain DS20 occupied a distinct phylogenetic position in the cluster, showing highest similarity with ‘’ IAM 12404 (98.8 %), followed by CC-FH12-1 (97.4 %) and IMMIB HF-1 (97.2 %). Therefore, the taxonomic characterization of ‘’ NBRC 16140 was also undertaken. Phylogenetic, chemotaxonomic and morphological analyses, based on signature sequences, DNA–DNA hybridizations, fatty acid profiles, physiological characterizations and polar lipid profiles confirmed that both strains DS20 and ’ NBRC 16140 represent two distinct species of the genus . Therefore, two novel species are proposed, sp. nov. (type strain, DS20=CCM 7540=MTCC 9471) and sp. nov. (type strain, NBRC 16140=IAM 12404=KCTC 2864).


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Comparative table of signature sequences for the genus and strains DS20 ( sp. nov.) and NBRC 16140 ( sp. nov.). [PDF](20 KB)


Fatty acid compositions of strains DS20 ( sp. nov.) and NBRC 16140 ( sp. nov.). [PDF](18 KB)


Polar lipid patterns for strains DS20 ( sp. nov.) and NBRC 16140 ( sp. nov.). [PDF](378 KB)


Transmission electron micrographs of cells of strains DS20 ( sp. nov.) and NBRC 16140 ( sp. nov.). [PDF](348 KB)

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