1887

Abstract

A total of 14 strains were isolated from the blood of two patients and from dialysis machines of a renal care centre. The strains were Gram-negative, halophilic, motile and non-spore-forming rods. They produced cream-coloured colonies and contained Q-9 as the predominant ubiquinone and C 7 and C as the major fatty acids. Phylogenetic analysis based on 16S rRNA gene sequencing showed that the 14 isolates were most closely related to 21 MI with 98.1–98.9 % sequence similarity and that they formed three separate lineages among themselves. Combined phenotypic and DNA–DNA hybridization data support the conclusion that they represent three novel species of the genus , for which the names sp. nov. (type strain S18214=KCTC 22148=DSM 21198), sp. nov. (type strain W1025=KCTC 22154=DSM 21196) and sp. nov. (type strain T68687=KCTC 22157=DSM 21197) are proposed.

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.004424-0
2010-02-01
2019-10-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/2/369.html?itemId=/content/journal/ijsem/10.1099/ijs.0.004424-0&mimeType=html&fmt=ahah

References

  1. Arahal, D. R. & Ventosa, A. ( 2006; ). The family Halomonadaceae. In The Prokaryotes: a Handbook on the Biology of Bacteria, 3rd edn, vol. 6, pp. 811–835. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. H. Schleifer & E. Stackebrandt. New York: Springer.
  2. Arahal, D. R., Ludwig, W., Schleifer, K. H. & Ventosa, A. ( 2002; ). Phylogeny of the family Halomonadaceae based on 23S and 16S rDNA sequence analyses. Int J Syst Evol Microbiol 52, 241–249.
    [Google Scholar]
  3. Ben Ali Gam, Z., Abdelkafi, S., Casalot, L., Tholozan, J. L., Oueslati, R. & Labat, M. ( 2007; ). Modicisalibacter tunisiensis gen. nov., sp. nov., an aerobic, moderately halophilic bacterium isolated from an oilfield-water injection sample, and emended description of the family Halomonadaceae Franzmann et al. 1989 emend. Dobson and Franzmann 1996 emend. Ntougias et al. 2007. Int J Syst Evol Microbiol 57, 2307–2313.[CrossRef]
    [Google Scholar]
  4. Ezaki, T., Hashimoto, Y. & Yabuuchi, E. ( 1989; ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef]
    [Google Scholar]
  5. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  6. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  7. Franzmann, P. D., Wehmeyer, U. & Stackebrandt, E. ( 1988; ). Halomonadaceae fam. nov., a new family of the class Proteobacteria to accommodate the genera Halomonas and Deleya. Syst Appl Microbiol 11, 16–19.[CrossRef]
    [Google Scholar]
  8. Gerhardt, P., Murray, R. G. E., Wood, W. A. & Krieg, N. R. (editors) ( 1994; ). Methods for General and Molecular Bacteriology. Washington, DC: American Society for Microbiology.
  9. Hall, T. A. ( 1999; ). BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41, 95–98.
    [Google Scholar]
  10. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  11. Kämpfer, P. & Kroppenstedt, R. M. ( 1996; ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef]
    [Google Scholar]
  12. Kaye, J. Z., Márquez, M. C., Ventosa, A. & Baross, J. A. ( 2004; ). Halomonas neptunia sp. nov., Halomonas sulfidaeris sp. nov., Halomonas axialensis sp. nov. and Halomonas hydrothermalis sp. nov.: halophilic bacteria isolated from deep-sea hydrothermal-vent environments. Int J Syst Evol Microbiol 54, 499–511.[CrossRef]
    [Google Scholar]
  13. Klatte, S., Rainey, F. A. & Kroppenstedt, R. M. ( 1994; ). Transfer of Rhodococcus aichiensis Tsukamura 1982 and Nocardia amarae Lechevalier and Lechevalier 1974 to the genus Gordona as Gordona aichiensis comb. nov. and Gordona amarae comb. nov. Int J Syst Bacteriol 44, 769–773.[CrossRef]
    [Google Scholar]
  14. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  15. Mata, J. A., Martínez-Cánovas, J., Quesada, E. & Béjar, V. ( 2002; ). A detailed phenotypic characterisation of the type strains of Halomonas species. Syst Appl Microbiol 25, 360–375.[CrossRef]
    [Google Scholar]
  16. McClung, L. S. & Toabe, R. ( 1947; ). The egg yolk plate reaction for the presumptive diagnosis of Clostridium sporogenes and certain species of the gangrene and botulinum groups. J Bacteriol 53, 139–147.
    [Google Scholar]
  17. NCCLS ( 2003; ). Performance Standards for Antimicrobial Disk Susceptibility Tests. Approved Standard, 8th edn. NCCLS document M2-A8 (ISBN 1-56238-485-6). Wayne, PA: National Committee for Clinical Laboratory Standards.
  18. Ntougias, S., Zervakis, G. I. & Fasseas, C. ( 2007; ). Halotalea alkalilenta gen. nov., sp. nov., a novel osmotolerant and alkalitolerant bacterium from alkaline olive mill wastes, and emended description of the family Halomonadaceae Franzmann et al. 1989, emend. Dobson and Franzmann 1996. Int J Syst Evol Microbiol 57, 1975–1983.[CrossRef]
    [Google Scholar]
  19. Ostle, A. G. & Holt, J. G. ( 1982; ). Nile blue A as a fluorescent stain for poly-β-hydroxybutyrate. Appl Environ Microbiol 44, 238–241.
    [Google Scholar]
  20. Peçonek, J., Gruber, C., Gallego, V., Ventosa, A., Busse, H.-J., Kämpfer, P., Radax, C. & Stan-Lotter, H. ( 2006; ). Reclassification of Pseudomonas beijerinckii Hof 1935 as Chromohalobacter beijerinckii comb. nov., and emended description of the species. Int J Syst Evol Microbiol 56, 1953–1957.[CrossRef]
    [Google Scholar]
  21. Rainey, F. A., Ward-Rainey, N., Kroppenstedt, R. M. & Stackebrandt, E. ( 1996; ). The genus Nocardiopsis represents a phylogenetically coherent taxon and a district actinomycete lineage: proposal of Nocardiopsaceae fam. nov. Int J Syst Bacteriol 46, 1088–1092.[CrossRef]
    [Google Scholar]
  22. Rodríguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1981; ). Characteristics of the heterotrophic bacterial population in hypersaline environments of different salt concentration. Microb Ecol 7, 235–243.[CrossRef]
    [Google Scholar]
  23. Romano, I., Giordano, A., Lama, L., Nicolaus, B. & Gambacorta, A. ( 2005; ). Halomonas campaniensis sp. nov., a haloalkaliphilic bacterium isolated from a mineral pool of Campania Region, Italy. Syst Appl Microbiol 28, 610–618.[CrossRef]
    [Google Scholar]
  24. Romano, I., Lama, L., Nicolaus, B., Poli, A., Gambacorta, A. & Giordano, A. ( 2006; ). Halomonas alkaliphila sp. nov., a novel halotolerant alkaliphilic bacterium isolated from a salt pool in Campania (Italy). J Gen Appl Microbiol 52, 339–348.[CrossRef]
    [Google Scholar]
  25. Saitou, N. & Nei, M. ( 1987; ). The neighbour-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  26. Sánchez-Porro, C., Tokunaga, H., Tokunaga, M. & Ventosa, A. ( 2007; ). Chromohalobacter japonicus sp. nov., a moderately halophilic bacterium isolated from a Japanese salty food. Int J Syst Evol Microbiol 57, 2262–2266.[CrossRef]
    [Google Scholar]
  27. Sánchez-Porro, C., de la Haba, R. R., Soto-Ramírez, N., Márquez, M. C., Montalvo-Rodríguez, R. & Ventosa, A. ( 2009; ). Description of Kushneria aurantia gen. nov., sp. nov., a novel member of the family Halomonadaceae, and a proposal for reclassification of Halomonas marisflavi as Kushneria marisflavi comb. nov., of Halomonas indalinina as Kushneria indalinina comb. nov. and of Halomonas avicenniae as Kushneria avicenniae comb. nov. Int J Syst Evol Microbiol 59, 397–405.[CrossRef]
    [Google Scholar]
  28. Sasser, M. ( 1990; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  29. Shin, Y. K., Lee, J.-S., Chun, C. O., Kim, H.-J. & Park, Y.-H. ( 1996; ). Isoprenoid quinone profiles of Leclercia adecarboxylata KCTC 1036T. J Microbiol Biotechnol 6, 68–69.
    [Google Scholar]
  30. Skerman, V. B. D. ( 1967; ). A Guide to the Identification of the Genera of Bacteria, 2nd edn. Baltimore: Williams & Wilkins.
  31. Stevens, D. A., Hamilton, J. R., Johnson, N., Kim, K. K. & Lee, J. S. ( 2008; ). Halomonas (H), newly recognized pathogen, causing infections and contamination in dialysis ctr. (DC); 3 new species. In Proceedings of the 48th Annual ICAAC/IDSA 46th Annual Meeting, abstract no. K-534, Washington, DC: American Society for Microbiology/Infectious Diseases Society of America.
  32. Tamaoka, J. & Komagata, K. ( 1984; ). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. FEMS Microbiol Lett 25, 125–128.[CrossRef]
    [Google Scholar]
  33. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  34. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A. ( 1982; ). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968.
    [Google Scholar]
  35. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  36. Wu, G., Wu, X.-Q., Wang, Y.-N., Chi, C.-Q., Tang, Y.-Q., Kida, K., Wu, X.-L. & Luan, Z.-K. ( 2008; ). Halomonas daqingensis sp. nov., a moderately halophilic bacterium isolated from an oilfield soil. Int J Syst Evol Microbiol 58, 2859–2865.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.004424-0
Loading
/content/journal/ijsem/10.1099/ijs.0.004424-0
Loading

Data & Media loading...

Phylogenetic tree based on 16S rRNA gene sequences, constructed using the neighbour-joining method, showing the positions of the novel strains among all recognized species of the genus . [ PDF] 91 KB

PDF

Combined File [ PDF] 59 KB

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error