Six obligately aerobic, Gram-negative, non-motile, pale-yellow-pigmented, rod-shaped bacterial strains, designated YM3-301, HG868, 04PA2 Co4-8B, 04PA2 Co4-99A, 04PA2 018SW-3 and 04PA2 018SW-18, were isolated from a marine sponge and seawater and were subjected to a polyphasic taxonomic investigation. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the novel isolates were affiliated with the family (phylum ) and that they showed the highest sequence similarity (90.3–90.9 %) to members of the genus and to . The 16S rRNA gene sequence similarities with respect to members of other related genera were only 86.3–89.1 %. In contrast, the six isolates shared high levels of 16S rRNA gene sequence similarity (99.7–100 %) and DNA-DNA relatedness (72–94 %) with each other. The novel isolates were phenotypically and physiologically different from members of related genera. The G+C content of the DNA was 41.8–43.5 mol%, MK-6 was the major menaquinone and i15 : 0, i15 : 1 and i17 : 0 3-OH were the major fatty acids. On the basis of the data from the taxonomic studies, it was concluded that these six novel strains represent a novel genus and species of the family , for which the name gen. nov., sp. nov. is proposed. The type strain of is YM3-301 (=KCTC 22160=MBIC 06275=NBRC 104141).


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vol. , part 2, pp. 207 - 212

Transmission electron micrograph of a cell of strain YM3-301 . [ PDF] 37 KB


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