The delineation of species among strains assigned to was examined using a gene genealogies-based approach in order to compare spliceosomal intron sequences found in four housekeeping genes (, , and ). This revealed four distinct groups of strains containing, respectively, var. CBS 767, var. CBS 789, var. CBS 1796 (the anamorph of var. CBS 789) and CBS 766, whose species status was no longer accepted. The sequence divergence between these groups, reaching in some cases over 20 %, unambiguously isolated the groups as separate taxa, leading to a proposal for the reinstatement of the originally described species CBS 767 and CBS 766. The variety var. was further subdivided into two taxa, CBS 789 and CBS 1796 (previously var. and ). The comparison of intron sequences therefore exposed cryptic species whose phenotypic traits are not distinguishable from known species, but which have significantly diverged from the genetic point of view. Hence, we describe the new taxon sp. nov. CBS 5571 is related to, but clearly distinct from, the species mentioned above. The approach used in this work has also revealed the existence of populations within the newly delineated species and genetic exchanges between these populations, indicating an unexpected genetic diversity within this part of the genus .


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vol. , part 5, pp. 1242–1251

List of oligonucleotides primers used in this study

Sequence divergence (%) within the gene intron between clades and species

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