A novel strain, designated MRN461, was isolated from a marine sponge in Micronesia. The 16S rRNA gene sequence of the isolate showed 95.6 % similarity with that of ‘’ IFO (now NBRC) 15994. Phylogenetic analysis revealed that the isolate and ‘’ IFO 15994 formed a distinct phyletic line within the family ‘’. Cells of strain MRN461 were Gram-negative, long filamentous rods, motile by gliding. Growth was observed at 15–40 °C (optimum, 33 °C), at pH 5.0–9.5 (optimum, pH 7.5) and in the presence of 0.5–7.0 % sea salts (optimum, 2.5 %). The major isoprenoid quinone was MK-7. The dominant fatty acids were summed feature 3 (comprising iso-C 2-OH and/or C 7; 34.8 %), C 5 (21.6 %) and iso-C (19.8 %). The DNA G+C content was 41.5 mol%. On the basis of evidence from our polyphasic taxonomic study, strain MRN461 is classified within a novel genus and species in the family ‘’, for which the name gen. nov., sp. nov. is proposed. The type strain of is strain MRN461 (=KCCM 42325 =JCM 14064). The misclassified species ‘[] ’ is transferred to the new genus as ( Lewin 1969) nom. rev., comb. nov., with LMG 13023 (=DSM 4134 =ATCC 23129 =NBRC 15994) as the type strain.


Article metrics loading...

Loading full text...

Full text loading...



  1. Bae, S. S., Lee, J.-H. & Kim, S.-J.(2005).Bacillus alveayuensis sp. nov., a thermophilic bacterium isolated from deep-sea sediments of the Ayu Trough. Int J Syst Evol Microbiol 55, 1211–1215.[CrossRef] [Google Scholar]
  2. Bae, S. S., Kwon, K. K., Yang, S.-H., Lee, H.-S., Kim, S.-J. & Lee, J. H.(2007).Flagellimonas eckloniae gen. nov., sp. nov., a mesophilic marine bacterium of the family Flavobacteriaceae, isolated from the rhizosphere of Ecklonia kurome. Int J Syst Evol Microbiol 57, 1050–1054.[CrossRef] [Google Scholar]
  3. Collins, M. D.(1985). Isoprenoid quinone analysis in classification and identification. In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by M. Goodfellow & D. E. Minnikin. London: Academic Press.
  4. Kämpfer, P., Young, C. C., Sridhar, K. R., Arun, A. B., Lai, W. A., Shen, F. T. & Rekha, P. D.(2006). Transfer of [Flexibacter] sancti, [Flexibacter] filiformis, [Flexibacter] japonensis and [Cytophaga] arvensicola to the genus Chitinophaga and description of Chitinophaga skermanii sp. nov. Int J Syst Evol Microbiol 56, 2223–2228.[CrossRef] [Google Scholar]
  5. Kwon, K. K., Lee, H.-S., Yang, S.-H. & Kim, S.-J.(2005).Kordiimonas gwangyangensis gen. nov., sp. nov., a marine bacterium isolated from marine sediments that forms a distinct phyletic lineage (Kordiimonadales ord. nov.) in the ‘Alphaproteobacteria’. Int J Syst Evol Microbiol 55, 2033–2037.[CrossRef] [Google Scholar]
  6. Lau, S. C. K., Tsoi, M. M. Y., Li, X., Plakhotnikova, I., Dobretsov, S., Wu, M., Wong, P.-K., Pawlik, J. R. & Qian, P.-Y.(2006). Description of Fabibacter halotolerans gen. nov., sp. nov. and Roseivirga spongicola sp. nov., and reclassification of [Marinicola] seohaensis as Roseivirga seohaensis comb. nov. Int J Syst Evol Microbiol 56, 1059–1065.[CrossRef] [Google Scholar]
  7. Lee, H.-S., Kwon, K. K., Yang, S.-H., Bae, S. S., Park, C. H., Kim, S.-J. & Lee, J.-H.(2008). Description of Croceitalea gen. nov. in the family Flavobacteriaceae with two species, Croceitalea eckloniae sp. nov. and Croceitalea dokdonensis sp. nov., isolated from the rhizosphere of the marine alga Ecklonia kurome. Int J Syst Evol Microbiol 58, 2505–2510.[CrossRef] [Google Scholar]
  8. Lewin, R. A.(1969). A classification of flexibacteria. J Gen Microbiol 58, 189–206.[CrossRef] [Google Scholar]
  9. Lewin, R. A. & Lounsbery, D. M.(1969). Isolation, cultivation and characterization of flexibacteria. J Gen Microbiol 58, 145–170.[CrossRef] [Google Scholar]
  10. Ludwig, W., Euzéby, J. & Whitman, W. B.(2008). Draft taxonomic outline of the Bacteroidetes, Planctomycetes, Chlamydiae, Spirochaetes, Fibrobacteres, Fusobacteria, Acidobacteria, Verrucomicrobia, Dictyoglomi, and Gemmatimonadetes. http://www.bergeys.org/outlines/Bergeys_Vol_4_Outline.pdf.
  11. Nakagawa, Y., Sakane, T., Suzuki, M. & Hatano, K.(2002). Phylogenetic structure of the genera Flexibacter, Flexithrix, and Microscilla deduced from 16S rRNA sequence analysis. J Gen Appl Microbiol 48, 155–165.[CrossRef] [Google Scholar]
  12. Nedashkovskaya, O. I., Suzuki, M., Vysotskii, M. V. & Mikhailov, V. V.(2003).Reichenbachia agariperforans gen. nov., sp. nov., a novel marine bacterium in the phylum CytophagaFlavobacteriumBacteroides. Int J Syst Evol Microbiol 53, 81–85.[CrossRef] [Google Scholar]
  13. Nedashkovskaya, O. I., Kim, S. B., Lee, D. H., Lysenko, A. M., Shevchenko, L. S., Frolova, G. M., Mikhailov, V. V., Lee, K. H. & Bae, K. S.(2005a).Roseivirga ehrenbergii gen. nov., sp. nov., a novel marine bacterium of the phylum ‘Bacteroidetes’ isolated from the green alga Ulva fenestrata. Int J Syst Evol Microbiol 55, 231–234.[CrossRef] [Google Scholar]
  14. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Park, M. S., Mikhailov, V. V., Bae, K. S. & Park, H. Y.(2005b).Roseivirga echinicomitans sp. nov., a novel marine bacterium isolated from the sea urchin Strongylocentrotus intermedius, and emended description of the genus Roseivirga. Int J Syst Evol Microbiol 55, 1797–1800.[CrossRef] [Google Scholar]
  15. Nedashkovskaya, O. I., Kim, S. B., Lysenko, A. M., Kalinovskaya, N. I. & Mikhailov, V. V.(2008). Reclassification of Roseivirga seohaensis (Yoon et al. 2005) Lau et al. 2006 as a later synonym of Roseivirga ehrenbergii Nedashkovskaya et al. 2005 and emendation of the species description. Int J Syst Evol Microbiol 58, 1194–1197.[CrossRef] [Google Scholar]
  16. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  17. Smibert, R. M. & Krieg, N. R.(1994). Phenotypic characterization. In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by P. Gerhardt, R. G. E. Murray, W. A. Wood & N. R. Krieg. Washington, DC: American Society for Microbiology.
  18. Sohn, J. H., Kwon, K. K., Kang, J.-H., Jung, H.-B. & Kim, S.-J.(2004).Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. Int J Syst Evol Microbiol 54, 1483–1487.[CrossRef] [Google Scholar]
  19. Stackebrandt, E. & Liesack, W.(1993). Nucleic acids and classification. In Handbook of New Bacterial Systematics, pp. 152–189. Edited by M. Goodfellow & A. G. O'Donnell. London: Academic Press.
  20. Takahashi, M., Suzuki, K. & Nakagawa, Y.(2006). Emendation of the genus Flammeovirga and Flammeovirga aprica with the proposal of Flammeovirga arenaria nom. rev., comb. nov. and Flammeovirga yaeyamensis sp. nov. Int J Syst Evol Microbiol 56, 2095–2100.[CrossRef] [Google Scholar]
  21. Yoon, J.-H., Kang, S.-J., Lee, C.-H. & Oh, T.-K.(2005).Marinicola seohaensis gen. nov., sp. nov., isolated from sea water of the Yellow Sea, Korea. Int J Syst Evol Microbiol 55, 859–863.[CrossRef] [Google Scholar]

Data & Media loading...


vol. , part 5, pp. 1204 - 1208

Differential characteristics of strain MRN 461 and '[ ] ' LMG 13023. [PDF](33 KB)

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error