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Abstract

A novel strain, designated MRN461, was isolated from a marine sponge in Micronesia. The 16S rRNA gene sequence of the isolate showed 95.6 % similarity with that of ‘’ IFO (now NBRC) 15994. Phylogenetic analysis revealed that the isolate and ‘’ IFO 15994 formed a distinct phyletic line within the family ‘’. Cells of strain MRN461 were Gram-negative, long filamentous rods, motile by gliding. Growth was observed at 15–40 °C (optimum, 33 °C), at pH 5.0–9.5 (optimum, pH 7.5) and in the presence of 0.5–7.0 % sea salts (optimum, 2.5 %). The major isoprenoid quinone was MK-7. The dominant fatty acids were summed feature 3 (comprising iso-C 2-OH and/or C 7; 34.8 %), C 5 (21.6 %) and iso-C (19.8 %). The DNA G+C content was 41.5 mol%. On the basis of evidence from our polyphasic taxonomic study, strain MRN461 is classified within a novel genus and species in the family ‘’, for which the name gen. nov., sp. nov. is proposed. The type strain of is strain MRN461 (=KCCM 42325 =JCM 14064). The misclassified species ‘[] ’ is transferred to the new genus as ( Lewin 1969) nom. rev., comb. nov., with LMG 13023 (=DSM 4134 =ATCC 23129 =NBRC 15994) as the type strain.

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2009-05-01
2019-12-05
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vol. , part 5, pp. 1204 - 1208

Differential characteristics of strain MRN 461 and '[ ] ' LMG 13023. [PDF](33 KB)



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