Two luminous marine bacteria, strains LC2-065 and LC2-102, were isolated from seawater at Sagami Bay in Japan. These bacteria were Gram-negative, oxidase-negative, catalase-positive, motile and coccoid-rods. 16S rRNA gene sequence analysis and multilocus sequence analysis (MLSA) using six loci (, , , , and ) and sequence analysis of the alpha subunit of luciferase () gene revealed that these bacteria were distinct from other species of the genus . These novel strains were most closely related to . The DNA–DNA hybridization value between strain LC2-065 and ATCC BAA-1194 was 42.1 %. The major fatty acids were C C, C, C and C iso 2-OH and/or C 7 (summed feature 3). The DNA G+C contents of strains LC2-065 and LC2-086 were 42.2 and 42.9 mol%, respectively. The phenotypic features of the novel strains were similar to those of and , but there were sufficient physiological differences for the novel strains to be easily differentiated. On the basis of these results, these new strains represent a novel species, for which the name sp. nov. is proposed. The type strain is LC2-065 (=NBRC 104633=KCTC 22356).


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Additional phylogenetic trees based on analysis of the 16S rRNA, , , , , and gene sequences. [ PDF] 211 KB


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