A Gram-negative, aerobic, neutrophilic, coccoid bacterium, strain E4A9, was isolated from a deep-sea sediment sample collected from the East Pacific polymetallic nodule region. 16S rRNA gene sequence analysis showed that the isolate was related to the type strain of (96.0 % sequence similarity). Lower 16S rRNA gene sequence similarities were observed with other members of the genera (94.7 %), (94.0–95.4 %), (94.8 %) and (94.6–95.1 %) of the family . Phylogenetic analysis including all described species of the family and several members of the family revealed that the isolate formed a distinct phylogenetic lineage with the family . Chemotaxonomic analysis revealed ubiquinone-10 as the predominant respiratory quinone, anteiso-C, iso-C and iso-C as major fatty acids, and phosphatidylglycerol as the major polar lipid. The DNA G+C content was 71.5 mol%. The isolate contained carotenoids, but no bacteriochlorophyll . On the basis of phenotypic and genotypic data presented in this study, strain E4A9 represents a novel species in a new genus in the family for which the name gen. nov., sp. nov. is proposed; the type strain is E4A9 (=CGMCC 1.6776=JCM 14846).


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vol. , part 9, pp. 2247 - 2253

Phylogenetic tree based on 16S rRNA gene sequences using the maximum-parsimony method.

Two-dimensional thin-layer chromatogram of the total polar lipids of strain E4A9 .

Absorption spectrum of strain E4A9 cells ultrasonically disrupted in MOPS buffer (a) and then extracted with acetone-methanol solution (b). The cells were grown under aerobic conditions in the light (1) or dark (2) conditions.

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