A moderately halophilic, Gram-positive-staining, endospore-forming, rod-shaped and strictly aerobic bacterium, designated strain NHBX5, was isolated from Lake Nanhuobuxun in China. Strain NHBX5 was able to grow at NaCl concentrations of 0–22 % (w/v) (optimally at 7 %, w/v), at pH 6.0–9.0 (optimally at pH 7.5) and at temperatures of 10–45 °C (optimally at 37 °C). The cell-wall peptidoglycan of strain NHBX5 contained -diaminopimelic acid as the diagnostic diamino acid. The predominant isoprenoid quinone was MK-7. The major cellular fatty acids were anteiso-C and anteiso-C. The polar lipids were phosphatidylglycerol, diphosphatidylglycerol and a glycolipid. The genomic DNA G+C content of strain NHBX5 was 44.9 mol%. Phylogenetic analysis based on 16S rRNA gene sequence comparisons revealed that strain NHBX5 was most closely related to EN8d (98.6 % gene sequence similarity). The level of DNA–DNA relatedness between strain NHBX5 and CGMCC 1.6199 was 34.6 %. Based on the data presented, strain NHBX5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NHBX5 (=DSM 19340 =CGMCC 1.6502). In addition, an emended description of the genus is presented.


Article metrics loading...

Loading full text...

Full text loading...



  1. Carrasco, I. J., Márquez, M. C., Xue, Y., Ma, Y., Cowan, D. A., Jones, B. E., Grant, W. D. & Ventosa, A.(2008).Sediminibacillus halophilus gen. nov., sp. nov., a moderately halophilic, Gram-positive bacterium from a hypersaline lake. Int J Syst Evol Microbiol 58, 1961–1967.[CrossRef] [Google Scholar]
  2. Collins, M. D., Pirouz, T., Goodfellow, M. & Minnikin, D. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. J Gen Microbiol 100, 221–230.[CrossRef] [Google Scholar]
  3. De Ley, J., Cattoir, H. & Reynaerts, A.(1970). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef] [Google Scholar]
  4. Doetsch, R. N.(1981). Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Philips. Washington, DC: American Society for Microbiology.
  5. Duckworth, A. W., Grant, W. D., Jones, B. E. & van Steenbergen, R.(1996). Phylogenetic diversity of soda lake alkaliphiles. FEMS Microbiol Ecol 19, 181–191.[CrossRef] [Google Scholar]
  6. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  7. Gregersen, T.(1978). Rapid method for distinction of Gram-negative from Gram-positive bacteria. Eur J Appl Microbiol Biotechnol 5, 123–127.[CrossRef] [Google Scholar]
  8. Groth, I., Schumann, P., Weiss, N., Martin, K. & Rainey, F. A.(1996).Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. Int J Syst Bacteriol 46, 234–239.[CrossRef] [Google Scholar]
  9. Huß, V. A. R., Festl, H. & Schleifer, K. H.(1983). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef] [Google Scholar]
  10. Kämpfer, P. & Kroppenstedt, R. M.(1996). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. Can J Microbiol 42, 989–1005.[CrossRef] [Google Scholar]
  11. Kates, M.(1986).Techniques of Lipidology, 2nd edn. Amsterdam: Elsevier.
  12. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  13. Kushner, D. J.(1985). The Halobacteriaceae. In The Bacteria, a Treatise on Structure and Function, vol. VIII, pp. 171–206. Edited by I. C. Gunsalus, C. R. Woese & R. S. Wolfe. San Diego: Academic Press.
  14. Mancinelli, R. L. & Hochstein, L. I.(1986). The occurrence of denitrification in extremely halophilic bacteria. FEMS Microbiol Lett 35, 55–58.[CrossRef] [Google Scholar]
  15. Marmur, J.(1961). A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3, 208–218.[CrossRef] [Google Scholar]
  16. Marmur, J. & Doty, P.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. J Mol Biol 5, 109–118.[CrossRef] [Google Scholar]
  17. Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: Microbial ID.
  18. Schleifer, K. H. & Kandler, O.(1972). Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev 36, 407–477. [Google Scholar]
  19. Sehgal, S. N. & Gibbons, N. E.(1960). Effect of some metal ions on the growth of Halobacterium cutirubrum. Can J Microbiol 6, 165–169.[CrossRef] [Google Scholar]
  20. Smibert, R. M. & Krieg, N. R.(1981). General characterization. In Manual of Methods for General Microbiology, pp. 409–443. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. B. Phillips. Washington, DC: American Society for Microbiology.
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  22. Ventosa, A., Quesada, E., Rodriguez-Valera, F., Ruiz-Berraquero, F. & Ramos-Cormenzana, A.(1982). Numerical taxonomy of moderately halophilic Gram-negative rods. J Gen Microbiol 128, 1959–1968. [Google Scholar]
  23. Ventosa, A., Nieto, J. J. & Oren, A.(1998). Biology of moderately halophilic aerobic bacteria. Microbiol Mol Biol Rev 62, 504–544. [Google Scholar]

Data & Media loading...


Scanning electron micrograph of cells of strain NHBX5 . Bar, 2.0 µm.


Cellular fatty acid profiles of strain NHBX5 and EN8d . [PDF](50 KB)

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error