A taxonomic study was carried out on strain B142, which was isolated from a crude-oil-degrading microbial consortium via enrichment with deep water from the Indian Ocean. Cells of the isolate were Gram-negative, oxidase-negative, catalase-positive, helical in shape, motile by means of polar flagella (three per cell) and moderately halophilic. Growth was observed at salinities of 0.5–12 % and at temperatures of 10–41 °C. The micro-organism was capable of denitrification, but was unable to degrade Tween 80 or gelatin. The predominant fatty acids were C 7 and/or iso-C2-OH (6.4 %), C (15.7 %), C 7 (45 %), C (6.8 %) and C 8 cyclo (6.7 %). The G+C content of the chromosomal DNA was 67.3 mol%. Comparisons of 16S rRNA gene sequences showed that strain B142 was most closely related to the type strains of two subspecies (93.0–93.1 % sequence similarity), two subspecies (92.8–92.9 %) and (91.7 %); sequence similarities with respect to other taxa were below 90.5 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain B142 formed a distinct evolutionary lineage within the family . Strain B142 was distinguishable from phylogenetically related genera with regard to several phenotypic properties. On the basis of phenotypic and phylogenetic data, therefore, strain B142 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is B142 (=CCTCC AB 208225=LMG 24627=MCCC 1A01235).


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Transmission electron micrographs of negatively stained cells of strain B142 . [PDF](271 KB)


Cellular fatty acid contents of strain B142 and related taxa. [PDF](21 KB)

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