1887

Abstract

A taxonomic study was carried out on strain B142, which was isolated from a crude-oil-degrading microbial consortium via enrichment with deep water from the Indian Ocean. Cells of the isolate were Gram-negative, oxidase-negative, catalase-positive, helical in shape, motile by means of polar flagella (three per cell) and moderately halophilic. Growth was observed at salinities of 0.5–12 % and at temperatures of 10–41 °C. The micro-organism was capable of denitrification, but was unable to degrade Tween 80 or gelatin. The predominant fatty acids were C 7 and/or iso-C2-OH (6.4 %), C (15.7 %), C 7 (45 %), C (6.8 %) and C 8 cyclo (6.7 %). The G+C content of the chromosomal DNA was 67.3 mol%. Comparisons of 16S rRNA gene sequences showed that strain B142 was most closely related to the type strains of two subspecies (93.0–93.1 % sequence similarity), two subspecies (92.8–92.9 %) and (91.7 %); sequence similarities with respect to other taxa were below 90.5 %. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain B142 formed a distinct evolutionary lineage within the family . Strain B142 was distinguishable from phylogenetically related genera with regard to several phenotypic properties. On the basis of phenotypic and phylogenetic data, therefore, strain B142 represents a novel genus and species, for which the name gen. nov., sp. nov. is proposed. The type strain is B142 (=CCTCC AB 208225=LMG 24627=MCCC 1A01235).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.003889-0
2009-06-01
2019-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/6/1278.html?itemId=/content/journal/ijsem/10.1099/ijs.0.003889-0&mimeType=html&fmt=ahah

References

  1. Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors) ( 1995; ). Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley.
  2. Gihring, T. M., Moser, D. P., Lin, L.-H., Davidson, M., Onstott, T. C., Morgan, L., Milleson, M., Kieft, T. L., Trimarco, E. & other authors ( 2006; ). The distribution of microbial taxa in the subsurface water of the Kalahari Shield, South Africa. Geomicrobiol J 23, 415–430.[CrossRef]
    [Google Scholar]
  3. Ivanova, E. P., Kiprianova, E. A., Mikhailov, V. V., Levanova, G. F., Garagulya, A. D., Gorshkova, N. M., Vysotskii, M. V., Nicolau, D. V., Yumoto, N. & other authors ( 1998; ). Phenotypic diversity of Pseudoalteromonas citrea from different marine habitats and emendation of the description. Int J Syst Bacteriol 48, 247–256.[CrossRef]
    [Google Scholar]
  4. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  5. Liu, C. & Shao, Z. ( 2005; ). Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol 55, 1181–1186.[CrossRef]
    [Google Scholar]
  6. Mesbah, M. & Whitman, W. B. ( 1989; ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr 479, 297–306.[CrossRef]
    [Google Scholar]
  7. Rzhetsky, A. & Nei, M. ( 1992; ). A simple method for estimating and testing minimum-evolution trees. Mol Biol Evol 9, 945–967.
    [Google Scholar]
  8. Rzhetsky, A. & Nei, M. ( 1993; ). Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol 10, 1073–1095.
    [Google Scholar]
  9. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  10. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  11. Sasser, M. ( 1997; ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
  12. Shieh, W. Y., Chen, Y.-W., Chaw, S.-M. & Chiu, H.-H. ( 2003; ). Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol 53, 479–484.[CrossRef]
    [Google Scholar]
  13. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  14. Yoon, J. H., Kang, S. J., Park, S. & Oh, T. K. ( 2007a; ). Caenispirillum bisanense gen. nov., sp. nov., isolated from sludge of a dye works. Int J Syst Evol Microbiol 57, 1217–1221.[CrossRef]
    [Google Scholar]
  15. Yoon, J. H., Kang, S. J., Park, S., Lee, S. Y. & Oh, T. K. ( 2007b; ). Reclassification of Aquaspirillum itersonii and Aquaspirillum peregrinum as Novispirillum itersonii gen. nov., comb. nov. and Insolitispirillum peregrinum gen. nov., comb. nov. Int J Syst Evol Microbiol 57, 2830–2835.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.003889-0
Loading
/content/journal/ijsem/10.1099/ijs.0.003889-0
Loading

Data & Media loading...

Supplements

Transmission electron micrographs of negatively stained cells of strain B142 . [PDF](271 KB)

PDF

Cellular fatty acid contents of strain B142 and related taxa. [PDF](21 KB)

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error