A novel mesophilic sulfate-reducing bacterium, EMSSDQ , was isolated from olive mill wastewater in the semi-arid region of Morocco (Marrakech). Cells were Gram-negative, catalase-positive, straight rods that were non-motile and non-spore-forming and contained cytochrome and desulfoviridin. The DNA G+C content was 65.1 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that the isolate was a member of the genus with D41, SPSN, JJ and EDK82 as the most closely related strains with validly published names. In addition to the classical substrates used by species, the isolate oxidized 1,4-tyrosol, one of the most abundant phenolic compounds occurring in olive mill wastewater, to 4-hydroxyphenylacetate without ring cleavage. SPSN was also found to carry out this reaction. Under air, strain EMSSDQ exhibited limited growth on lactate and yeast extract in the absence of sulfate. On the basis of genotypic and phenotypic characteristics, it is proposed that the isolate represents a novel species, sp. nov. The type strain is EMSSDQ (=DSM 19337 =ATCC BAA-1562).


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Phase-contrast photomicrograph (a) and transmission electron photomicrograph (b), showing the absence of flagella, of cells of strain EMSSDQ grown on 10 mM lactate as carbon and energy source in the presence of 20 mM sulfate as electron acceptor and 0.1 g yeast extract l . Phase-contrast photomicroscopy was performed as previously described (Qatibi , 1991) using a photomicroscope (Nikon Eclipse E600) with an oil-immersion objective (×100). Transmission electron photomicroscopy was carried out at the Department of Microbiology, Institute of Biological Sciences, University of Aarhus, Denmark, according to Jakobsen (2006). Bars, 10 µm (a) and 2 µm (b).


Growth of strain EMSSDQ , DSM 5433 , DSM 3604 and DSM 3852 under air in the absence of sulfate. [PDF](26 KB)

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