A bacterial strain designated SBKo001 was isolated from a forest soil sample from Mt Makiling in Laguna, Philippines. It shows the general characteristics associated with myxobacteria, such as swarming of Gram-negative, rod-shaped vegetative cells, fruiting body formation and bacteriolytic activity. The strain is mesophilic, strictly aerobic and chemoheterotrophic and also exhibits resistance to various antibiotics. Major fatty acids are iso-C, C 2-OH and C (arachidonic acid). The G+C content of the genomic DNA is 69.2 mol%. A reference strain, NOSO-1 (=DSM 53757), isolated from the Etosha Basin in Namibia, shares nearly the same characteristics with SBKo001. The identical 16S rRNA gene sequences of the two strains show 94 % identity to strains of the cellulose-degrading and species. Phylogenetic analysis reveals a novel branch diverging from the , , . Their uniqueness in morphological growth stages, unusual fatty acid profile, broad-spectrum antibiotic resistance and branch divergence from the imply that strains SBKo001 and NOSO-1 not only represent a novel genus and species, proposed here as gen. nov., sp. nov., but also belong to a new family, fam. nov. The type strain of is SBKo001 (=DSM 21295 =NCCB 100230).


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Swarm colony of SBKo001 . (a) Five-day-old colony on MD1 agar. Bar, 2 cm. (b) Radiating fine veins from the agar inoculum, on PM-12 agar. Bar, 500 µm. (c) Flame-like edges and developing mounds of cells (arrow) on P-12 agar. Bar, 300 µm.


Dark granules located at poles of vegetative cells of strain SBKo001 . Bar, 12 µm.


Thick-walled sporangioles (arrows) of strain SBKo001 . (a) Fluorescent micrograph; (b) laser-scanning micrograph. Bars, 20 µm (a) and 40 µm (b).


Phase-contrast micrographs of fruiting bodies of strains NOSO-1 (a) and SBKo001 (b) on VY/2 agar. Bars, 50 µm.

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