A methanogenic organism from the domain (SD1) was isolated from saline water released from a coal seam located 926 m below the surface via a methane-producing well near Monroe, Louisiana, USA. Growth and methanogenesis were supported with methanol, monomethylamine, dimethylamine or trimethylamine, but not with dimethylsulfide, formate, acetate or H/CO. Cells grew in high-salt minimal medium but growth was stimulated with yeast extract or tryptone. Cells were single, non-motile, irregular coccoids 0.5–1.0 μm in diameter and the cell wall contained protein. Conditions for the maximum rate of growth were 40–50 °C, 0.2–0.6 M NaCl, 100–≥200 mM MgCl, and pH 7.0–8.0. The G+C content of the genomic DNA was 42±1mol %. A comparison of 16S rRNA gene sequences indicated that strain SD1 was most closely related to DSM 5435 with 96 % gene sequence similarity. It is proposed that strain SD1 represents a novel species, sp. nov. The type strain is SD1 (=ATCC BAA-1601=DSM 21339).

Keyword(s): CBM, coal bed methane

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vol. , part 5, pp. 1064 - 1069

Factors influencing the growth rate of sp. nov. SD1T. [ PDF]36 KB

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