Two strains of nocardioform actinomycetes, isolated from soil and plant litter in Yamanashi prefecture, Japan, showed substrate mycelium with purple to dark-red colours. 16S rRNA gene sequence analysis indicated that the organisms belonged to the family and were related closely to strains (96.7–98.0 % sequence similarity). The isolates contained MK-9(H) as the predominant menaquinone, -diaminopimelic acid, galactose as the diagnostic whole-cell sugar and phosphatidylethanolamine as the predominant polar lipid. Based on a combination of cultural, physiological and chemotaxonomic properties, in addition to the phylogenetic analysis and DNA–DNA hybridization data, we propose a novel species, sp. nov., for these strains, with the type strain YU 692-1 (=NBRC 102064 =KCTC 19326).


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  1. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  2. Felsenstein, J.(1985). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef] [Google Scholar]
  3. Gordon, R. E., Barnett, D. A., Handerhan, J. E. & Pang, C. H.-N.(1974).Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. Int J Syst Bacteriol 24, 54–63.[CrossRef] [Google Scholar]
  4. Hayakawa, M. & Nonomura, H.(1987). Humic acid–vitamin agar, a new medium for selective isolation of soil actinomycetes. J Ferment Technol 65, 501–509.[CrossRef] [Google Scholar]
  5. Hu, Y.-T., Zhou, P.-J., Zhou, Y.-G., Liu, Z.-H. & Liu, S.-J.(2004).Saccharothrix xinjigangensis sp. nov., a pyrene-degrading actinomycete isolated from Tianchi Lake, Xinjigang, China. Int J Syst Evol Microbiol 54, 2091–2094.[CrossRef] [Google Scholar]
  6. Kumar, S., Tamura, K. & Nei, M.(2004).mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef] [Google Scholar]
  7. Labeda, D. P.(2001).Crossiella gen. nov., a new genus related to Streptoalloteichus. Int J Syst Evol Microbiol 51, 1575–1579. [Google Scholar]
  8. Labeda, D. P.(2002). The family Actinosynnemataceae. In The Prokaryotes, 3rd edn, pp. 654–668. Edited by M. Dworkin, S. Falkow, E. Rosenberg, K. Schleifer & E. Stackebrandt. New York: Springer.
  9. Labeda, D. P. & Kroppenstedt, R. M.(2006).Goodfellowia gen. nov., a new genus of the Pseudonocardineae related to Actinoalloteichus, containing Goodfellowia coeruleoviolacea gen. nov., comb. nov. Int J Syst Evol Microbiol 56, 1203–1207.[CrossRef] [Google Scholar]
  10. Labeda, D. P. & Kroppenstedt, R. M.(2007). Proposal of Umezawaea gen. nov., a new genus of the Actinosynnemataceae related to Saccharothrix, and transfer of Saccharothrixtangerinus Kinoshita et al. 2000 as Umezawaeatangerina gen. nov., comb. nov. Int J Syst Evol Microbiol 57, 2758–2761.[CrossRef] [Google Scholar]
  11. Labeda, D. P., Testa, R. T., Lechevalier, M. P. & Lechevalier, H. A.(1984).Saccharothrix: a new genus of the Actinomycetales related to Nocardiopsis. Int J Syst Bacteriol 34, 426–431.[CrossRef] [Google Scholar]
  12. Labeda, D. P., Hatano, K., Kroppenstedt, R. M. & Tamura, T.(2001). Revival of the genus Lentzea and proposal for Lechevalieria gen. nov. Int J Syst Evol Microbiol 51, 1045–1050.[CrossRef] [Google Scholar]
  13. Lechevalier, M. P. & Lechevalier, H. A.(1970). Chemical composition as a criterion in the classification of aerobic actinomycetes. Int J Syst Bacteriol 20, 435–443.[CrossRef] [Google Scholar]
  14. Lechevalier, M. P., Stern, A. E. & Lechevalier, H. A.(1977). Chemotaxonomy of aerobic actinomycetes: phospholipid composition. Biochem Syst Ecol 5, 249–260.[CrossRef] [Google Scholar]
  15. Saito, H. & Miura, K.(1963). Preparation of transforming deoxyribonucleic acid by phenol treatment. Biochim Biophys Acta 72, 619–629.[CrossRef] [Google Scholar]
  16. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  17. Shirling, E. B. & Gottlieb, D.(1966). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef] [Google Scholar]
  18. Takahashi, K. & Nei, M.(2000). Efficiencies of fast algorithms of phylogenetic inference under the criteria of maximum parsimony, minimum evolution and maximum likelihood when a large number of sequences are used. Mol Biol Evol 17, 1251–1258.[CrossRef] [Google Scholar]
  19. Tamura, T. & Hatano, K.(2001). Phylogenetic analysis of the genus Actinoplanes and transfer of Actinoplanesminutisporangius Ruan et al. 1986 and ‘Actinoplanesaurantiacus’ to Cryptosporangiumminutisporangium comb. nov. and Cryptosporangiumaurantiacum sp. nov. Int J Syst Evol Microbiol 51, 2119–2125.[CrossRef] [Google Scholar]
  20. Tamura, T., Nakagaito, Y., Nishii, T., Hasegawa, T., Stackebrandt, E. & Yokota, A.(1994). A new genus of the order Actinomycetales, Couchioplanes gen. nov., with descriptions of Couchioplanes caeruleus (Horan and Brodsky 1986) comb. nov. and Couchioplanes caeruleus subsp. azureus subsp. nov. Int J Syst Bacteriol 44, 193–203.[CrossRef] [Google Scholar]
  21. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  22. Williams, S. T., Goodfellow, M., Alderson, G., Wellington, E. M. H., Sneath, P. H. A. & Sackin, M. J.(1983). Numerical classification of Streptomyces and related taxa. J Gen Microbiol 129, 1743–1813. [Google Scholar]
  23. Yassin, A. F., Rainey, F. A., Brzezinka, H., Jahnke, K. D., Weissbrodt, H., Budzikiewicz, H., Stackebrandt, E. & Schaal, K. P.(1995).Lentzea gen. nov., a new genus of the order Actinomycetales. Int J Syst Bacteriol 45, 357–363.[CrossRef] [Google Scholar]

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vol. , part 6, pp. 1504 – 1507

Scanning electron micrograph of the synnemata of strain YU 692-1 grown on HV agar for 2 weeks at 28 °C. Bar, 10 µm.

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