1887

Abstract

Although and , two genera of thermophilic bacteria close to the genus , have only been described recently, the number of species in these genera has increased rapidly. Four thermophilic, lipolytic strains (DR01, DR02, DR03 and DR04) isolated from a hot spring in Veracruz (Mexico), which could not be identified phenotypically, were subjected to 16S rRNA gene sequence analysis. Three strains were identified as belonging to the genus , but strain DR03 was identified as . This result led us to perform a phylogenetic analysis of the genera and based on 16S rRNA gene sequences from all the type strains of these genera. Phylogenetic trees showed three major clusters, , and , while the 16S rRNA gene sequences of (DR03 and the type strain) showed low similarity to sequences of (92.5–95.1 %) and (92.8–94.5 %) species, as well as to (92.2–92.4 %). In addition, could be differentiated from and on the basis of DNA G+C content and fatty acid and polar lipid profiles. From these results, it is proposed that should be classified in a novel genus, for which we propose the name , as gen. nov., comb. nov. The type strain of is H12 (=ATCC 51176 =DSM 3670 =LMG 19006).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.003699-0
2010-07-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/60/7/1600.html?itemId=/content/journal/ijsem/10.1099/ijs.0.003699-0&mimeType=html&fmt=ahah

References

  1. Banat, I. M., Marchant, R. & Rahman, T. J. ( 2004; ). Geobacillus debilis sp. nov., a novel obligately thermophilic bacterium isolated from a cool soil environment, and reassignment of Bacillus pallidus to Geobacillus pallidus comb. nov. Int J Syst Evol Microbiol 54, 2197–2201.[CrossRef]
    [Google Scholar]
  2. Castro-Ochoa, L. D., Rodríguez-Gómez, C., Valerio-Alfaro, G. & Ros, R. O. ( 2005; ). Screening, purification and characterization of the thermoalkalophilic lipase produced by Bacillus thermoleovorans CCR11. Enzyme Microb Technol 37, 648–654.[CrossRef]
    [Google Scholar]
  3. Derekova, A., Sjøholm, C., Mandeva, R. & Kambourova, M. ( 2007; ). Anoxybacillus rupiensis sp. nov., a novel thermophilic bacterium isolated from Rupi basin (Bulgaria). Extremophiles 11, 577–583.[CrossRef]
    [Google Scholar]
  4. Gillis, M., Vandamme, P., De Vos, P., Swings, J. & Kersters, K. ( 2001; ). Polyphasic taxonomy. In Bergey's Manual of Systematic Bacteriology, 2nd edn, vol. 1, pp. 43–48. Edited by G. M. Garrity, D. R. Boone & R. W. Castenholz. New York: Springer.
  5. Guindon, S. & Gascuel, O. ( 2003; ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol 52, 696–704.[CrossRef]
    [Google Scholar]
  6. Kämpfer, P., Rosselló-Mora, R., Falsen, E., Busse, H.-J. & Tindall, B. J. ( 2006; ). Cohnella thermotolerans gen. nov., sp. nov., and classification of ‘Paenibacillus hongkongensis’ as Cohnella hongkongensis sp. nov. Int J Syst Evol Microbiol 56, 781–786.[CrossRef]
    [Google Scholar]
  7. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  8. Miñana-Galbis, D., Farfán, M., Fusté, M. C. & Lorén, J. G. ( 2007; ). Aeromonas bivalvium sp. nov., isolated from bivalve molluscs. Int J Syst Evol Microbiol 57, 582–587.[CrossRef]
    [Google Scholar]
  9. Nazina, T. N., Tourova, T. P., Poltaraus, A. B., Novikova, E. V., Grigoryan, A. A., Ivanova, A. E., Lysenko, A. M., Petrunyaka, V. V., Osipov, G. A. & other authors ( 2001; ). Taxonomic study of aerobic thermophilic bacilli: descriptions of Geobacillus subterraneus gen. nov., sp. nov. and Geobacillus uzenensis sp. nov. from petroleum reservoirs and transfer of Bacillus stearothermophilus, Bacillus thermocatenulatus, Bacillus thermoleovorans, Bacillus kaustophilus, Bacillus thermoglucosidasius and Bacillus thermodenitrificans to Geobacillus as the new combinations G. stearothermophilus, G. thermocatenulatus, G. thermoleovorans, G. kaustophilus, G. thermoglucosidasius and G. thermodenitrificans. Int J Syst Evol Microbiol 51, 433–446.
    [Google Scholar]
  10. Nazina, T. N., Lebedeva, E. V., Poltaraus, A. B., Tourova, T. P., Grigoryan, A. A., Sokolova, D. S., Lysenko, A. M. & Osipov, G. A. ( 2004; ). Geobacillus gargensis sp. nov., a novel thermophile from a hot spring, and the reclassification of Bacillus vulcani as Geobacillus vulcani comb. nov. Int J Syst Evol Microbiol 54, 2019–2024.[CrossRef]
    [Google Scholar]
  11. Nazina, T. N., Sokolova, D. S., Grigoryan, A. A., Shestakova, N. M., Mikhailova, E. M., Poltaraus, A. B., Tourova, T. P., Lysenko, A. M., Osipov, G. A. & Belyaev, S. S. ( 2005; ). Geobacillus jurassicus sp. nov., a new thermophilic bacterium isolated from a high-temperature petroleum reservoir, and the validation of the Geobacillus species. Syst Appl Microbiol 28, 43–53.[CrossRef]
    [Google Scholar]
  12. Pikuta, E., Lysenko, A., Chuvilskaya, N., Mendrock, U., Hippe, H., Suzina, N., Nikitin, D., Osipov, G. & Laurinavichius, K. ( 2000; ). Anoxybacillus pushchinensis gen. nov., sp. nov., a novel anaerobic, alkaliphilic, moderately thermophilic bacterium from manure, and description of Anoxybacillus flavithermus comb. nov. Int J Syst Evol Microbiol 50, 2109–2117.[CrossRef]
    [Google Scholar]
  13. Pikuta, E., Cleland, D. & Tang, J. ( 2003; ). Aerobic growth of Anoxybacillus pushchinoensis K1T: emended descriptions of A. pushchinoensis and the genus Anoxybacillus. Int J Syst Evol Microbiol 53, 1561–1562.[CrossRef]
    [Google Scholar]
  14. Pinzón, D., Miñana, D., Manresa, A., Valerio, G., García, H. & Oliart-Ros, R. ( 2007a; ). Isolation of new thermophilic lipases from Veracruz's hot springs. In Abstracts of the International Conference on Enzyme Technology “Relatenz 2007”, 20–23 June 2007, Varadero, Cuba.
  15. Pinzón, D., Miñana, D., García, H., Valerio, G. & Oliart, R. ( 2007b; ). Obtención de lipasas a partir de ambientes termales de Veracruz. In Abstracts of the XII Congreso Nacional de Biotecnología y Bioingeniería, 25–29 June 2007, Morelia, Mexico. Poster CIV-122. León, Guanajuato, Mexico: IV Encuentro ‘Participación de la Mujer en la Ciencia’ (in Spanish).
  16. Poli, A., Esposito, E., Lama, L., Orlando, P., Nicolaus, G., de Appolonia, F., Gambacorta, A. & Nicolaus, B. ( 2006; ). Anoxybacillus amylolyticus sp. nov., a thermophilic amylase producing bacterium isolated from Mount Rittmann (Antarctica). Syst Appl Microbiol 29, 300–307.[CrossRef]
    [Google Scholar]
  17. Rahman, R. N. Z. R. A., Leow, T. C., Salleh, A. B. & Basri, M. ( 2007; ). Geobacillus zalihae sp. nov., a thermophilic lipolytic bacterium isolated from palm oil mill effluent in Malaysia. BMC Microbiol 7, 77 [CrossRef]
    [Google Scholar]
  18. Schmidt-Dannert, C., Sztajer, H., Stöcklein, W., Menge, U. & Schmid, R. D. ( 1994; ). Screening, purification and properties of a thermophilic lipase from Bacillus thermocatenulatus. Biochim Biophys Acta 1214, 43–53.[CrossRef]
    [Google Scholar]
  19. Scholz, T., Demharter, W., Hensel, R. & Kandler, O. ( 1987; ). Bacillus pallidus sp. nov., a new thermophilic species from sewage. Syst Appl Microbiol 9, 91–96.[CrossRef]
    [Google Scholar]
  20. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  21. Tindall, B. J. ( 1990; ). Lipid composition of Halobacterium lacusprofundi. FEMS Microbiol Lett 66, 199–202.[CrossRef]
    [Google Scholar]
  22. Tindall, B. J., Sikorski, J., Smibert, R. A. & Krieg, N. R. ( 2007; ). Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Bacteriology, pp. 330–393. Edited by C. A. Reddy, T. J. Beveridge, T. A. Breznak, G. A. Marzluf, T. M. Schmidt & L. R. Snyder. Washington, DC: American Society for Microbiology.
  23. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  24. Zeigler, D. R. ( 2005; ). Application of a recN sequence similarity analysis to the identification of species within the bacterial genus Geobacillus. Int J Syst Evol Microbiol 55, 1171–1179.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.003699-0
Loading
/content/journal/ijsem/10.1099/ijs.0.003699-0
Loading

Data & Media loading...

Supplements

Pairwise sequence similarity (%) between 16S rRNA gene sequences of type strains of and species. [Excel file](27 KB)

EXCEL

DNA G+C contents and cellular fatty acid compositions of and species. [PDF](64 KB)

PDF

Maximum-likelihood phylogenetic tree obtained from 33 sequences of the 16S rRNA gene, encompassing all and species. [PDF](44 KB)

PDF

Polar lipid profiles after separation by two-dimensional TLC from DSM 12423 (a), LMG 19006 (b) and CECT 43 (c). DPG, Diphosphatidylglycerol; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; AL1, unknown aminolipid; APL1–2, unknown aminophospholipid; GPL1, unknown glycophospholipid; GL1–3, unknown glycolipid; PL1, unknown phospholipid; L, unknown polar lipid.

IMAGE

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error