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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 59, Issue 4

sp. nov., a keratin-degrading actinobacterium isolated from bovine manure compost Free

Abstract

A novel keratinolytic actinobacterium, strain WCC-2265, was isolated from bovine hoof keratin ‘baited’ into composting bovine manure from southern Alberta, Canada, and subjected to phenotypic and genotypic characterization. Strain WCC-2265 produced well-developed, non-fragmenting and extensively branched hyphae within substrates and aerial hyphae, from which spherical spores possessing spiny cell sheaths were produced in primarily flexuous or straight chains. The cell wall contained -diaminopimelic acid, whole-cell sugars were galactose, glucose, madurose and ribose, and the major menaquinones were MK-9(H), MK-9(H), MK-9(H) and MK-9(H). These characteristics suggested that the organism belonged to the genus and a comparative analysis of 16S rRNA gene sequences indicated that it formed a distinct clade within the genus. Strain WCC-2265 could be differentiated from other species of the genus by DNA–DNA hybridization, morphological and physiological characteristics and the predominance of iso-C, iso-C and 10-methyl C fatty acids. The broad range of phenotypic and genetic characters supported the suggestion that this organism represents a novel species of the genus , for which the name sp. nov. is proposed; the type strain is strain WCC-2265 (=DSM 45195=CCUG 56181).

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Keyword(s): SEM, scanning electron microscopy
© Minister of Public Works and Government Services Canada
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2009-04-01
2024-04-23
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Actinomadura keratinilytica sp. nov., a keratin-degrading actinobacterium isolated from bovine manure compost
Int J Syst Evol Microbiol 59, 828 (2009); https://doi.org/10.1099/ijs.0.003640-0
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Strain WCC-2265 grown on keratin agar for 5 days at 45 °C displaying its ability to degrade keratin within the medium. [ PDF] 152 KB

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Cellular fatty acid profiles of strain WCC-2265 , , and the type species . [ PDF] 43 KB

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