1887

Abstract

A novel Gram-negative, non-sporulating, moderately halophilic, facultatively alkaliphilic, catalase- and oxidase-positive, obligately aerobic bacterium, strain YIM-Y25, was isolated from a subterranean brine sample collected from a salt mine in Yunnan, south-west China. Cells were spirilla, motile by monopolar flagella, with -diaminopimelic acid in the cell-wall peptidoglycan. Growth occurred with 1–15 % (w/v) NaCl (optimum 5 %), at pH 6.0–10.0 (optimum pH 8.0) and at 15–50 °C (optimum 35–40 °C). Ubiquinone Q-8 was the predominant respiratory quinone. Polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylmonomethylamine and an unidentified phospholipid. The major cellular fatty acids were C 7, iso-C, C and C. The genomic DNA G+C content was 58.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM-Y25 was most closely related to the type strain of the sole recognized species of the genus , EL-105 (sequence similarity 97.0 %), and these two strains formed a robust lineage in the phylogenetic tree. The level of DNA–DNA relatedness between them was 12.6 %. The combination of phylogenetic analysis, phenotypic differences, chemotaxonomic properties and DNA–DNA hybridization data supported the view that this strain represents a novel species of the genus , for which the name sp. nov. is proposed, with YIM-Y25 (=CCTCC AA 207033 =KCTC 22169) as the type strain.

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2009-06-01
2019-10-21
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