The name ‘’ has been used previously to designate three marine hydrocarbon-degrading strains, of which strain RAG-1 (=ATCC 31012) has industrial applications for the production of the bioemulsifier emulsan. However, to date, the name of this taxon has not been validly published. In this study, five strains were examined to corroborate the delineation of this taxon by means of phenotypic characterization, DNA–DNA hybridization, selective restriction fragment amplification (AFLP), amplified rDNA restriction analysis (ARDRA), gene sequence analysis and tRNA intergenic spacer length polymorphism analysis (tDNA-PCR) and to emend the description of ‘’ ( Di Cello 1997 ). AFLP analysis showed that the five strains formed a tight cluster at 56.8±5.0 % genomic relatedness that was separated from strains of other haemolytic species of the genus and from the type and reference strains of other species at ≤27 % relatedness, indicating the distinctiveness of the novel strains. The strains were haemolytic and able to grow on citrate (Simmons), -histidine and malonate. The strains did not oxidize -glucose or utilize -lactate or -aspartate. The G+C contents of strains RAG-1 and of VE-C3 were 43.9 % and 43.6 mol%, respectively. The novel strains could be recognized by a characteristic ARDRA pattern (I 1, I 3, I 2, I 2, I 3). The consensus tDNA-PCR pattern for the five strains consisted of amplified fragments of 87.9, 100.2, 134.6 and 248.5 bp and was indistinguishable from that of strains of genomic species 14BJ. The five strains represent a novel species for which the name sp. nov. is proposed. The type strain is RAG-1 (=ATCC 31012=CCUG 45561=LMG 19082=LUH 3904=NIPH 1925).


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Growth of different strains after 72 h in mineral medium containing carbon sources with variable chain lengths. [ PDF] 62 KB


-values of the differences in delta yield over the range of carbon sources used between the various strains and the control strain RAG-1 . [ PDF] 35 KB

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