1887

Abstract

Dissimilatory iron reduction plays a significant role in subsurface environments. Currently, it is assumed that members of the genus constitute the majority of the iron-reducing micro-organisms that oxidize aromatic compounds in contaminated subsurface environments. Here, we report the isolation of two phylogenetically distinct pure cultures of iron-reducing degraders of monoaromatic hydrocarbons, strain TMJ1, which belongs to the genus within the , and strain UKTL, belonging to the genus within the . Both strains utilize a wide range of substrates as carbon and energy sources, including the aromatic compounds toluene, phenol and -cresol. Additionally, strain UKTL utilizes -xylene and TMJ1 utilizes -cresol. Anaerobic degradation of toluene in both strains and -xylene in strain UKTL is initiated by activation with fumarate addition to the methyl group. The genomic DNA G+C contents of strains TMJ1 and UKTL are 54.4 and 47.7 mol%, respectively. Based on a detailed physiological characterization and phylogenetic analysis of the 16S rRNA genes of both strains, we propose the names sp. nov. (type strain UKTL =DSM 19510 =JCM 15765) and sp. nov. (type strain TMJ1 =DSM 19350 =JCM 15764) to accommodate these strains. To the best of our knowledge, strain UKTL is the first described spore-forming, iron-reducing bacterium that can degrade aromatic hydrocarbons.

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2010-03-01
2019-10-18
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Electron micrographs of strain TMJ1 grown on acetate-fumarate freshwater medium (a) and strain UKTL grown on toluene-ferric citrate medium (b). Bars, 1 µm.

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Electron micrographs of strain TMJ1 grown on acetate-fumarate freshwater medium (a) and strain UKTL grown on toluene-ferric citrate medium (b). Bars, 1 µm.

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Main morphological and physiological characteristics of strain TMJ1 and its closest relatives within the genus (Table S1) and strain UKTL and its closest relatives in the genera and (Table S2). [PDF](88 KB)

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