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A Gram-negative, heterotrophic, aerobic, pale orange-pigmented, non-endospore-forming and motile bacterial strain, designated strain SP8T, was isolated from a salty water sample from the solar salterns of Santa Pola, located on the Mediterranean coast of Spain. The strain grew optimally at 37 °C, pH 6.5 and in the presence of 10 % NaCl. A polyphasic taxonomic study was conducted in order to characterize the strain in detail. Phylogenetic analyses based on 16S rRNA gene sequence comparisons indicated that strain SP8T clustered within the branch constituted by species of the genus Halomonas. The closest phylogenetic neighbours of strain SP8T were Halomonas muralis LMG 20969T (96.0 % sequence similarity), Halomonas pantelleriensis AAPT (95.9 %) and Halomonas campaniensis 5AGT (95.8 %). Phenotypic features, the fatty acid profile and the DNA G+C content of the novel strain further supported its placement in the genus Halomonas. On the basis of phenotypic, chemotaxonomic and phylogenetic distinctiveness, it is suggested that strain SP8T represents a novel species for which the name Halomonas ilicicola sp. nov. is proposed. The type strain is SP8T (=CECT 7331T=CCM 7522T=DSM 19980T).
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International Journal of Systematic and Evolutionary Microbiology vol. 59 , part 3, pp. 578 - 582
Supplementary Fig. S1. Phylogenetic trees, based on neighbour-joining and maximum-likelihood analysis of the 16S rRNA gene sequences, showing the position of strain SP8 Tand closely related species. [ PDF] 53 KB