1887

Abstract

Five independent strains, isolated from clinical samples but probably not responsible for disease, revealed phenotypic and genotypic features that appeared to exclude their belonging to any of the recognized species. The strains, which are non-pigmented rapid growers, presented a cell-wall lipid pattern resembling those of and . Sequencing of the 16S rRNA, and genes and the 16S–23S rRNA gene internal transcribed spacer (ITS) revealed that the strains are clearly distinct from every other species. While the 16S rRNA and genes were characterized by a single sequevar, two sequevars were detected in and three in the ITS. The divergence shown in the latter region was striking, in which only two short regions (less than 150 bp in all) were comparable with other mycobacteria, apart from and . The PCR restriction analysis pattern of the novel strains also differs from any reported to date. The name sp. nov. is proposed for the novel species; the type strain is FI-06250 (=DSM 45132 =CIP 109609).

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2009-06-01
2019-10-20
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vol. , part 6, pp. 1518 - 1523

Fatty acid compositions of three strains of sp. nov. determined by GLC.

Neighbour-joining phylogenetic trees based on (Fig. S1) and (Fig. S2) sequences.

[PDF file of Supplementary Table and Figures](617 KB)



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