A taxonomic study was carried out on a Gram-negative, rod-shaped, non-motile, halophilic bacterium, designated strain W11-2BT, which was isolated from a pyrene-degrading consortium that was enriched from sediment from the Pacific Ocean. Growth was observed at salinities of 0.5–10 % and at temperatures of 10–41 °C. Strain W11-2BT was unable to degrade Tween 80 or gelatin. 16S rRNA gene sequence comparisons showed that strain W11-2BT was related most closely to Oceanicola nanhaiensis SS011B1-20T (95.8 % similarity) and Oceanicola batsensis HTCC2597T (95.7 %); levels of 16S rRNA gene sequence similarity between strain W11-2BT and the type strains of other species tested were below 95.2 %. The dominant fatty acids of strain W11-2BT were C18 : 1ω7c (32.1 % of the total), C19 : 0 cyclo (20.9 %), C18 : 1ω7c 11-methyl (19.5 %), C18 : 0 (7.3 %), C17 : 0 (6.6 %) and C16 : 0 (3.8 %). The G+C content of the chromosomal DNA was 64.6 mol%. The above data were in good agreement with those of members of the genus Oceanicola. Based on morphology, physiology, fatty acid composition and 16S rRNA gene sequence data, strain W11-2BT is considered to represent a novel species of the genus Oceanicola, for which the name Oceanicola pacificus sp. nov. is proposed. The type strain is W11-2BT (=CCTCC AB 208224T=LMG 24619T=MCCC 1A01034T).
Ausubel, F. M., Brent, R., Kingston, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (editors)(1995).Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, 3rd edn. New York: Wiley.
Cho, J.-C. & Giovannoni, S. J.(2004).Oceanicola granulosus gen. nov., sp. nov. and Oceanicola batsensis sp. nov., poly-β-hydroxybutyrate-producing marine bacteria in the order ‘Rhodobacterales’. Int J Syst Evol Microbiol54, 1129–1136.[CrossRef][Google Scholar]
Gu, J., Guo, B., Wang, Y.-N., Yu, S.-L., Inamori, R., Qu, R., Ye, Y.-G. & Wu, X.-L.(2007).Oceanicola nanhaiensis sp. nov., isolated from sediments of the South China Sea. Int J Syst Evol Microbiol57, 157–160.[CrossRef][Google Scholar]
Lin, K. Y., Sheu, S. Y., Chang, P. S., Cho, J. C. & Chen, W. M.(2007).Oceanicola marinus sp. nov., a marine alphaproteobacterium isolated from seawater collected off Taiwan. Int J Syst Evol Microbiol57, 1625–1629.[CrossRef][Google Scholar]
Liu, C. L. & Shao, Z. Z.(2005).Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. Int J Syst Evol Microbiol55, 1181–1186.[CrossRef][Google Scholar]
Mesbah, M. & Whitman, W. B.(1989). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine+cytosine of DNA. J Chromatogr479, 297–306.[CrossRef][Google Scholar]
Rzhetsky, A. & Nei, M.(1992). A simple method for estimating and testing minimum evolution trees. Mol Biol Evol9, 945–967.
[Google Scholar]
Rzhetsky, A. & Nei, M.(1993). Theoretical foundation of the minimum-evolution method of phylogenetic inference. Mol Biol Evol10, 1073–1095.
[Google Scholar]
Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol4, 406–425.
[Google Scholar]
Sambrook, J., Fritsch, E. F. & Maniatis, T.(1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
Sasser, M.(1990).Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE: MIDI Inc.
Shieh, W. Y., Chen, Y.-W., Chaw, S.-M. & Chiu, H.-H.(2003).Vibrio ruber sp. nov., a red, facultatively anaerobic, marine bacterium isolated from sea water. Int J Syst Evol Microbiol53, 479–484.[CrossRef][Google Scholar]
Stackebrandt, E. & Goebel, B. M.(1994). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol44, 846–849.[CrossRef][Google Scholar]
Tamura, K., Dudley, J., Nei, M. & Kumar, S.(2007).mega4: molecular evolutionary genetics analysis (mega) software version 4.0. Mol Biol Evol24, 1596–1599.[CrossRef][Google Scholar]
Wang, B. J., Lai, Q. L., Cui, Z. S., Tan, T. F. & Shao, Z. Z.(2008). A pyrene-degrading consortium from deep-sea sediment of the west pacific and its key member Cycloclasticus sp. P1. Environ Microbiol10, 1948–1963.[CrossRef][Google Scholar]