1887

Abstract

An alkaliphilic actinobacterium, designated strain CAAS 252, was isolated from the black liquor treatment system of a cotton pulp mill in Wuhan, China. Cells of strain CAAS 252 were Gram-positive, non-motile, non-endospore-forming, short rod-shaped, and grew optimally at 42 °C and pH 9–10 in the presence of 3 % (w/v) NaCl. Strain CAAS 252 contained MK-7, MK-8 and MK-9 as the major menaquinones and anteiso-C, anteiso-C and C as the predominant cellular fatty acids and had a peptidoglycan type of A4, Lys–Gly--Asp. The DNA G+C content was 60.2 mol%. Based on analysis of 16S rRNA gene sequences (94.7–96.8 % similarity), DNA–DNA hybridization (<70 % relatedness) and chemotaxonomic characteristics, strain CAAS 252 belonged to the genus , but differed from all recognized species. Therefore, it is proposed that strain CAAS 252 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CAAS 252 (=CCTCC AB 207011=DSM 19423).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.003376-0
2009-04-01
2019-12-14
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/4/863.html?itemId=/content/journal/ijsem/10.1099/ijs.0.003376-0&mimeType=html&fmt=ahah

References

  1. Collins, M. D., Lawson, P. A., Labrenz, M., Tindall, B. J., Weiss, N. & Hirsch, P. ( 2002; ). Nesterenkonia lacusekhoensis sp. nov., isolated from hypersaline Ekho Lake, East Antarctica, and emended description of the genus Nesterenkonia. Int J Syst Evol Microbiol 52, 1145–1150.[CrossRef]
    [Google Scholar]
  2. Cowan, S. T. & Steel, K. J. ( 1965; ). Manual for the Identification of Medical Bacteria. London: Cambridge University Press.
  3. De Ley, J. ( 1970; ). Reexamination of the association between melting point, buoyant density, and chemical base composition of deoxyribonucleic acid. J Bacteriol 101, 738–754.
    [Google Scholar]
  4. De Ley, J., Cattoir, H. & Reynaerts, A. ( 1970; ). The quantitative measurement of DNA hybridization from renaturation rates. Eur J Biochem 12, 133–142.[CrossRef]
    [Google Scholar]
  5. Delgado, O., Quillaguamán, J., Bakhtiar, S., Mattiasson, B., Gessesse, A. & Hatti-Kaul, R. ( 2006; ). Nesterenkonia aethiopica sp. nov., an alkaliphilic, moderate halophile isolated from an Ethiopian soda lake. Int J Syst Evol Microbiol 56, 1229–1232.[CrossRef]
    [Google Scholar]
  6. Fitch, W. M. ( 1971; ). Toward defining the course of evolution: minimum change for a specific tree topology. Syst Zool 20, 406–416.[CrossRef]
    [Google Scholar]
  7. Huß, V. A. R., Festl, H. & Schleifer, K. H. ( 1983; ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. Syst Appl Microbiol 4, 184–192.[CrossRef]
    [Google Scholar]
  8. Jukes, T. H. & Cantor, C. R. ( 1969; ). Evolution of protein molecules. In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by H. N. Munro. New York: Academic Press.
  9. Lányí, B. ( 1987; ). Classical and rapid identification methods for medically important bacteria. Methods Microbiol 19, 1–67.
    [Google Scholar]
  10. Leifson, E. ( 1963; ). Determination of carbohydrate metabolism of marine bacteria. J Bacteriol 85, 1183–1184.
    [Google Scholar]
  11. Li, W.-J., Chen, H.-H., Zhang, Y.-Q., Schumann, P., Stackebrandt, E., Xu, L.-H. & Jiang, C.-L. ( 2004; ). Nesterenkonia halotolerans sp. nov. and Nesterenkonia xinjiangensis sp. nov., actinobacteria from saline soils in the west of China. Int J Syst Evol Microbiol 54, 837–841.[CrossRef]
    [Google Scholar]
  12. Li, W.-J., Chen, H.-H., Kim, C.-J., Zhang, Y.-Q., Park, D.-J., Lee, J.-C., Xu, L.-H. & Jiang, C.-L. ( 2005; ). Nesterenkonia sandarakina sp. nov. and Nesterenkonia lutea sp. nov., novel actinobacteria, and emended description of the genus Nesterenkonia. Int J Syst Evol Microbiol 55, 463–466.[CrossRef]
    [Google Scholar]
  13. Li, W.-J., Zhang, Y.-Q., Schumann, P., Liu, H.-Y., Yu, L.-Y., Zhang, Y.-Q., Stackebrandt, E., Xu, L.-H. & Jiang, C.-L. ( 2008; ). Nesterenkonia halophila sp. nov., a novel moderately halophilic and alkalitolerant actinobacterium isolated from a saline soil. Int J Syst Evol Microbiol 58, 1359–1363.[CrossRef]
    [Google Scholar]
  14. Lu, G. & Moriyama, E. N. ( 2004; ). Vector NTI, a balanced all-in-one sequence analysis suite. Brief Bioinform 5, 378–388.[CrossRef]
    [Google Scholar]
  15. Luo, H.-Y., Miao, L.-H., Fang, C., Yang, P.-L., Wang, Y.-R., Shi, P.-J., Yao, B. & Fan, Y.-L. ( 2008; ). Nesterenkonia flava sp. nov., isolated from paper mill effluent. Int J Syst Evol Microbiol 58, 1927–1930.[CrossRef]
    [Google Scholar]
  16. Rzhetsky, A. & Nei, M. ( 1992; ). Statistical properties of the ordinary least-squares, generalized least-squares, and minimum-evolution methods of phylogenetic inference. J Mol Evol 35, 367–375.[CrossRef]
    [Google Scholar]
  17. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  18. Shirling, E. B. & Gottlieb, D. ( 1966; ). Methods for characterization of Streptomyces species. Int J Syst Bacteriol 16, 313–340.[CrossRef]
    [Google Scholar]
  19. Stackebrandt, E. & Goebel, B. M. ( 1994; ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. Int J Syst Bacteriol 44, 846–849.[CrossRef]
    [Google Scholar]
  20. Stackebrandt, E., Koch, C., Gvozdiak, O. & Schumann, P. ( 1995; ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. Int J Syst Bacteriol 45, 682–692.[CrossRef]
    [Google Scholar]
  21. Tamura, K., Dudley, J., Nei, M. & Kumar, S. ( 2007; ). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. Mol Biol Evol 24, 1596–1599.[CrossRef]
    [Google Scholar]
  22. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  23. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors ( 1987; ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef]
    [Google Scholar]
  24. Yoon, J.-H., Jung, S.-Y., Kim, W., Nam, S.-W. & Oh, T.-K. ( 2006; ). Nesterenkonia jeotgali sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. Int J Syst Evol Microbiol 56, 2587–2592.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.003376-0
Loading
/content/journal/ijsem/10.1099/ijs.0.003376-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error