@article{mbs:/content/journal/ijsem/10.1099/ijs.0.003368-0, author = "Noar, Jesse D. and Buckley, Daniel H.", title = "Ideonella azotifigens sp. nov., an aerobic diazotroph of the Betaproteobacteria isolated from grass rhizosphere soil, and emended description of the genus Ideonella", journal= "International Journal of Systematic and Evolutionary Microbiology", year = "2009", volume = "59", number = "8", pages = "1941-1946", doi = "https://doi.org/10.1099/ijs.0.003368-0", url = "https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.003368-0", publisher = "Microbiology Society", issn = "1466-5034", type = "Journal Article", keywords = "PW-PCR, plate-wash PCR", abstract = "Strain 1a22T, a nitrogen-fixing bacterium, was isolated from soil associated with the rhizosphere of a perennial grass growing in a fallow agricultural field in Ithaca, New York, USA. Analysis of the 16S rRNA gene sequence placed the strain in the Rubrivivax–Roseateles–Leptothrix–Azohydromonas–Aquincola–Ideonella branch of the Betaproteobacteria and the closest characterized relative was the type strain of Ideonella dechloratans (97.7 % 16S rRNA sequence similarity). Cells of strain 1a22T were Gram-negative, motile, straight rods, which formed polyhydroxybutyrate-like granules and were positive for oxidase and weakly positive for catalase. Cells were chemo-organotrophic, unable to grow by reduction of chlorate or nitrate and grew exclusively through aerobic respiration. Growth with mannitol on N-free solid media caused the strain to produce copious amounts of slime. The G+C content of the genomic DNA was 67.4 mol%. The major cellular fatty acids were C16 : 1 cis-9 and C16 : 0 and cells contained significant amounts of the hydroxy fatty acids C10 : 0 3-OH, C12 : 0 2-OH and C12 : 0 3-OH. Based on DNA–DNA hybridization studies, 16S rRNA gene sequence analysis, fatty acid analysis, and morphological and physiological characteristics, strain 1a22T represents a novel species in the genus Ideonella, for which the name Ideonella azotifigens sp. nov. is proposed. The type strain of Ideonella azotifigens is 1a22T (=JCM 15503T=DSM 21438T).", }