Strain 1a22, a nitrogen-fixing bacterium, was isolated from soil associated with the rhizosphere of a perennial grass growing in a fallow agricultural field in Ithaca, New York, USA. Analysis of the 16S rRNA gene sequence placed the strain in the branch of the and the closest characterized relative was the type strain of (97.7 % 16S rRNA sequence similarity). Cells of strain 1a22 were Gram-negative, motile, straight rods, which formed polyhydroxybutyrate-like granules and were positive for oxidase and weakly positive for catalase. Cells were chemo-organotrophic, unable to grow by reduction of chlorate or nitrate and grew exclusively through aerobic respiration. Growth with mannitol on N-free solid media caused the strain to produce copious amounts of slime. The G+C content of the genomic DNA was 67.4 mol%. The major cellular fatty acids were C -9 and C and cells contained significant amounts of the hydroxy fatty acids C 3-OH, C 2-OH and C 3-OH. Based on DNA–DNA hybridization studies, 16S rRNA gene sequence analysis, fatty acid analysis, and morphological and physiological characteristics, strain 1a22 represents a novel species in the genus , for which the name sp. nov. is proposed. The type strain of is 1a22 (=JCM 15503=DSM 21438).


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