1887

Abstract

Strain 10-1-101, isolated from a sand sample collected from the desert of Xinjiang, China, was a Gram-negative, aerobic, motile, rod-shaped bacterium. Colonies grown on 0.1× trypticase soy broth agar were circular, convex and light-pink-coloured. The major cellular fatty acids of the novel strain were C 7 (72.71 %) and C (7.05 %). The DNA GC content of strain 10-1-101was 68.8 mol% and Q-10 was the major respiratory quinone. 16S rRNA gene sequence analysis indicated that strain 10-1-101 was related to type strains of the genus , with sequence similarity values of 94.07 % with DSM 18479 and 92.74 % with DSM 9527. On the basis of genotypic, phenotypic and phylogenetic data, this new strain represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 10-1-101 (=CCTCC AB 207153=NRRL B-51273).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.003350-0
2009-06-01
2019-12-07
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/6/1531.html?itemId=/content/journal/ijsem/10.1099/ijs.0.003350-0&mimeType=html&fmt=ahah

References

  1. Burris, R. H. ( 1972; ). Nitrogen fixation – assay methods and techniques. Methods Enzymol 24, 415–431.
    [Google Scholar]
  2. Cappuccino, J. G. & Sherman, N. ( 2002; ). Microbiology: a Laboratory Manual, 6th edn. Menlo Park, CA: Benjamin Cummings.
  3. Chanal, A., Chapon, V., Benzerara, K., Barakat, M., Christen, R., Achouak, W., Barras, F. & Heulin, T. ( 2006; ). The desert of Tataouine: an extreme environment that hosts a wide diversity of microorganisms and radiotolerant bacteria. Environ Microbiol 8, 514–525.[CrossRef]
    [Google Scholar]
  4. Chun, J., Lee, J.-H., Jung, Y., Kim, M., Kim, S., Kim, B. K. & Lim, Y.-W. ( 2007; ). EzTaxon: a web-based tool for the identification of prokaryotes based on 16S ribosomal RNA gene sequences. Int J Syst Evol Microbiol 57, 2259–2261.[CrossRef]
    [Google Scholar]
  5. Doetsch, R. N. ( 1981; ). Determinative methods of light microscopy. In Manual of Methods for General Bacteriology, pp. 21–33. Edited by P. Gerhardt, R. G. E. Murray, R. N. Costilow, E. W. Nester, W. A. Wood, N. R. Krieg & G. H. Phillips. Washington, DC: American Society for Microbiology.
  6. Eckert, B., Weber, O. B., Kirchhof, G., Halbritter, A., Stoffels, M. & Hartmann, A. ( 2001; ). Azospirillum doebereinerae sp. nov., a nitrogen fixing bacterium associated with the C4-grass Miscanthus. Int J Syst Evol Microbiol 51, 17–26.
    [Google Scholar]
  7. Felsenstein, J. ( 1985; ). Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.[CrossRef]
    [Google Scholar]
  8. Golyshina, O. V., Pivovarova, T. A., Karavaiko, G. I., Kondratéva, T. F., Moore, E. R., Abraham, W. R., Lünsdorf, H., Timmis, K. N., Yakimov, M. M. & Golyshin, P. N. ( 2000; ). Ferroplasma acidiphilum gen. nov., sp. nov., an acidophilic, autotrophic, ferrous-iron-oxidizing, cell-wall-lacking, mesophilic member of the Ferroplasmaceae fam. nov., comprising a distinct lineage of the Archaea. Int J Syst Evol Microbiol 50, 997–1006.[CrossRef]
    [Google Scholar]
  9. Hopwood, D. A., Bibb, M. J., Chater, K. F., Kieser, T., Bruton, C. J., Kieser, H. M., Lydiate, D. J., Smith, C. P., Ward, J. M. & Schrempf, H. (editors) ( 1985; ). Genetic Manipulation of Streptomyces. A Laboratory Manual. Norwich: John Innes Foundation.
  10. Kimura, M. ( 1980; ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol 16, 111–120.[CrossRef]
    [Google Scholar]
  11. Kumar, S., Tamura, K. & Nei, M. ( 2004; ). mega 3: integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5, 150–163.[CrossRef]
    [Google Scholar]
  12. Lin, Y.-C., Uemori, K., de Briel, D. A., Arunpairojana, V. & Yokota, A. ( 2004; ). Zimmermannella helvola gen. nov., sp. nov., Zimmermannella alba sp. nov., Zimmermannella bifida sp. nov., Zimmermannella faecalis sp. nov. and Leucobacter albus sp. nov., novel members of the family Microbacteriaceae. Int J Syst Evol Microbiol 54, 1669–1676.[CrossRef]
    [Google Scholar]
  13. Mesbah, M., Premachandran, U. & Whitman, W. B. ( 1989; ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. Int J Syst Bacteriol 39, 159–167.[CrossRef]
    [Google Scholar]
  14. Saitou, N. & Nei, M. ( 1987; ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425.
    [Google Scholar]
  15. Skerman, V. B. D., Sly, L. I. & Williamson, M.-L. ( 1983; ). Conglomeromonas largomobilis gen. nov., sp. nov., a sodium-sensitive, mixed-flagellated organism from fresh waters. Int J Syst Bacteriol 33, 300–308.[CrossRef]
    [Google Scholar]
  16. Sly, L. I. & Stackebrandt, E. ( 1999; ). Description of Skermanella parooensis gen. nov., sp. nov. to accommodate Conglomeromonas largomobilis subsp. parooensis following the transfer of Conglomeromonas largomobilis subsp. largomobilis to the genus Azospirillum. Int J Syst Bacteriol 49, 541–544.[CrossRef]
    [Google Scholar]
  17. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G. ( 1997; ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef]
    [Google Scholar]
  18. Wagner-Döbler, I., Rheims, H., Felske, A., El-Ghezal, A., Flade-Schröder, D., Laatsch, H., Lang, S., Pukall, R. & Tindall, B. J. ( 2004; ). Oceanibulbus indolifex gen. nov., sp. nov., a North Sea alphaproteobacterium that produces bioactive metabolites. Int J Syst Evol Microbiol 54, 1177–1184.[CrossRef]
    [Google Scholar]
  19. Weon, H.-Y., Kim, B.-Y., Hong, S.-B., Joa, J.-H., Nam, S.-S., Lee, K.-H. & Kwon, S.-W. ( 2007; ). Skermanella aerolata sp. nov., isolated from air, and emended description of the genus Skermanella. Int J Syst Evol Microbiol 57, 1539–1542.[CrossRef]
    [Google Scholar]
  20. Xie, C. H. & Yokota, A. ( 2003; ). Phylogenetic analysis of Lampropedia hyalina based on the 16S rRNA gene sequence. J Gen Appl Microbiol 49, 345–349.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.003350-0
Loading
/content/journal/ijsem/10.1099/ijs.0.003350-0
Loading

Data & Media loading...

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error