Skip to content
1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 59, Issue 4

sp. nov., a metal-resistant symbiont of growing on metallicolous soil in Languedoc, France Free

Abstract

A polyphasic taxonomic approach was used to characterize 31 rhizobial isolates obtained from , a metallicolous legume species, growing close to a zinc mine in the south of France (Saint Laurent le Minier). Comparative analysis of nearly full-length 16S rRNA gene sequences showed that these Gram-negative bacteria belonged to the genus and that they were related most closely to ORS 2640. The phylogenetic relationships of these isolates with other species were confirmed by sequencing and analysis of the and genes, which were used as alternative chromosomal markers. These novel mesorhizobial strains tolerated high concentrations of heavy metals: 16–32 mM Zn and 0.3–0.5 mM Cd. DNA–DNA hybridizations revealed >73 % relatedness between the strains isolated from , but only 19–33 % relatedness between these and the type strains of and . These results, together with other phenotypic characteristics, support the conclusion that these isolates represent a single, novel species of the genus , for which the name sp. nov. is proposed. The type strain is STM 2683 (=CFBP 7147=LMG 24485).

  • Published Online:
© SGM
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.003327-0
2009-04-01
2025-03-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/59/4/850.html?itemId=/content/journal/ijsem/10.1099/ijs.0.003327-0&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J.(1990). Basic local alignment search tool. J Mol Biol 215, 403–410.[CrossRef] [Google Scholar]
  2. Andrews, J. M.(2001). Determination of minimum inhibitory concentrations. J Antimicrob Chemother 48, 5–16.[CrossRef] [Google Scholar]
  3. Bertrand, H., Plassard, C., Pinochet, X., Touraine, B., Normand, P. & Cleyet-Marel, J.-C.(2000). Stimulation of the ionic-transport system in Brassica napus by a plant growth-promoting rhizobacterium (Achromobacter sp.). Can J Microbiol 46, 229–236.[CrossRef] [Google Scholar]
  4. Cervantes, C. & Gutierrez-Corona, F.(1994). Copper resistance mechanisms in bacteria and fungi. FEMS Microbiol Rev 14, 121–137. [Google Scholar]
  5. Chen, W. X., Li, G. S., Qi, Y. L., Wang, E. T., Yuan, H. L. & Li, J. L.(1991).Rhizobium huakuii sp. nov. isolated from the root nodules of Astragalus sinicus. Int J Syst Bacteriol 41, 275–280.[CrossRef] [Google Scholar]
  6. Chen, W., Wang, E., Wang, S., Li, Y. & Chen, X.(1995). Characteristics of Rhizobium tianshanense sp. nov., a moderately and slowly growing root nodule bacterium isolated from an arid saline environment in Xinjiang, People's Republic of China. Int J Syst Bacteriol 45, 153–159.[CrossRef] [Google Scholar]
  7. de Lajudie, P., Willems, A., Nick, G., Moreira, F., Molouba, F., Hoste, B., Torck, U., Neyra, M., Collins, M. D. & other authors(1998). Characterization of tropical tree rhizobia and description of Mesorhizobium plurifarium sp. nov. Int J Syst Bacteriol 48, 369–382.[CrossRef] [Google Scholar]
  8. Ezaki, T., Hashimoto, Y. & Yabuuchi, E.(1989). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. Int J Syst Bacteriol 39, 224–229.[CrossRef] [Google Scholar]
  9. Frérot, H., Lefèbvre, C., Gruber, W., Collin, C., Dos Santos, A. & Escarré, J.(2006). Specific interactions between local metallicolous plants improve the phytostabilization of mine soils. Plant Soil 282, 53–65.[CrossRef] [Google Scholar]
  10. Gao, J.-L., Turner, S. L., Kan, F. L., Wang, E. T., Tan, Z. Y., Qiu, Y. H., Gu, J., Terefework, Z., Young, J. P. & other authors(2004).Mesorhizobium septentrionale sp. nov. and Mesorhizobium temperatum sp. nov., isolated from Astragalus adsurgens growing in the northern regions of China. Int J Syst Evol Microbiol 54, 2003–2012.[CrossRef] [Google Scholar]
  11. Gaunt, M. W., Turner, S. L., Rigottier-Gois, L., Lloyd-Macgilp, S. A. & Young, J. P. W.(2001). Phylogenies of atpD and recA support the small subunit rRNA-based classification of rhizobia. Int J Syst Evol Microbiol 51, 2037–2048.[CrossRef] [Google Scholar]
  12. Ghosh, W. & Roy, P.(2006).Mesorhizobium thiogangeticum sp. nov., a novel sulfur-oxidizing chemolithoautotroph from rhizosphere soil of an Indian tropical leguminous plant. Int J Syst Evol Microbiol 56, 91–97.[CrossRef] [Google Scholar]
  13. Jarvis, B. D. W., Pankhurst, C. E. & Patel, J. J.(1982).Rhizobium loti, a new species of legume root nodule bacteria. Int J Syst Bacteriol 32, 378–380.[CrossRef] [Google Scholar]
  14. Jarvis, B. D. W., van Berkum, P., Chen, W. X., Nour, S. M., Fernandez, M. P., Cleyet-Marel, J. C. & Gillis, M.(1997). Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov. Int J Syst Bacteriol 47, 895–898.[CrossRef] [Google Scholar]
  15. Kwon, S.-W., Park, J.-Y., Kim, J.-S., Kang, J.-W., Cho, Y.-H., Lim, C.-K., Parker, M. A. & Lee, G.-B.(2005). Phylogenetic analysis of the genera Bradyrhizobium, Mesorhizobium, Rhizobium and Sinorhizobium on the basis of 16S rRNA gene and internally transcribed spacer region sequences. Int J Syst Evol Microbiol 55, 263–270.[CrossRef] [Google Scholar]
  16. Nicholas, K. B. & Nicholas, H. B.(1997). GeneDoc: a tool for editing and annotating multiple sequence alignments, v2.6.001. Distributed by the authors. http://www.nrbsc.org/gfx/genedoc/
  17. Nies, D. H.(2003). Efflux-mediated heavy metal resistance in prokaryotes. FEMS Microbiol Rev 27, 313–339.[CrossRef] [Google Scholar]
  18. Normand, P., Cournoyer, B., Simonet, P. & Nazaret, S.(1992). Analysis of a ribosomal RNA operon in the actinomycete Frankia. Gene 111, 119–124.[CrossRef] [Google Scholar]
  19. Nour, S. M., Fernandez, M. P., Normand, P. & Cleyet-Marel, J. C.(1994).Rhizobium ciceri sp. nov., consisting of strains that nodulate chickpeas (Cicer arietinum L.). Int J Syst Bacteriol 44, 511–522.[CrossRef] [Google Scholar]
  20. Nour, S. M., Cleyet-Marel, J. C., Normand, P. & Fernandez, M. P.(1995). Genomic heterogeneity of strains nodulating chickpeas (Cicer arietinum L.) and description of Rhizobium mediterraneum sp. nov. Int J Syst Bacteriol 45, 640–648.[CrossRef] [Google Scholar]
  21. Page, R. D.(1996). TreeView: an application to display phylogenetic trees on personal computers. Comput Appl Biosci 12, 357–358. [Google Scholar]
  22. Posada, D. & Crandall, K.(1998).modeltest: testing the model of DNA substitution. Bioinformatics 14, 817–818.[CrossRef] [Google Scholar]
  23. Saitou, N. & Nei, M.(1987). The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4, 406–425. [Google Scholar]
  24. Swofford, D. L.(1998).paup: Phylogenetic analysis using parsimony (and other methods). Sunderland, MA: Sinauer Associates.
  25. Thompson, J. D., Gibson, T. J., Plewniak, F., Jeanmougin, F. & Higgins, D. G.(1997). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25, 4876–4882.[CrossRef] [Google Scholar]
  26. Velazquez, E., Igual, J., Willems, A., Fernández, M. P., Muñoz, E., Mateos, P. F., Abril, A., Toro, N., Normand, P. & other authors(2001).Mesorhizobium chacoense sp. nov., a novel species that nodulates Prosopis alba in the Chaco Arido region (Argentina). Int J Syst Evol Microbiol 51, 1011–1021.[CrossRef] [Google Scholar]
  27. Vincent, J. M.(1970). The cultivation, isolation and maintenance of rhizobia. In A Manual for the Practical Study of the Root-Nodule Bacteria, pp. 1–13. Edited by J. M. Vincent. Oxford: Blackwell Scientific.
  28. Vinuesa, P., Silva, C., Lorite, M. J., Izaguirre-Mayoral, M. L., Bedmar, E. J. & Martinez-Romero, E.(2005). Molecular systematics of rhizobia based on maximum likelihood and Bayesian phylogenies inferred from rrs, atpD, recA and nifH sequences, and their use in the classification of Sesbania microsymbionts from Venezuelan wetlands. Syst Appl Microbiol 28, 702–716.[CrossRef] [Google Scholar]
  29. Wang, E. T., van Berkum, P., Sui, X. H., Beyene, D., Chen, W. X. & Martinez-Romero, E.(1999). Diversity of rhizobia associated with Amorpha fruticosa isolated from Chinese soils and description of Mesorhizobium amorphae sp. nov. Int J Syst Bacteriol 49, 51–65.[CrossRef] [Google Scholar]
  30. Wang, F. Q., Wang, E. T., Liu, J., Chen, Q., Sui, X. H., Chen, W. F. & Chen, W. X.(2007).Mesorhizobium albiziae sp. nov., a novel bacterium that nodulates Albizia kalkora in a subtropical region of China. Int J Syst Evol Microbiol 57, 1192–1199.[CrossRef] [Google Scholar]
  31. Wayne, L. G., Brenner, D. J., Colwell, R. R., Grimont, P. A. D., Kandler, O., Krichevsky, M. I., Moore, L. H., Moore, W. E. C., Murray, R. G. E. & other authors(1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. Int J Syst Bacteriol 37, 463–464.[CrossRef] [Google Scholar]
  32. Weir, B. S., Turner, S. J., Silvester, W. B., Park, D.-C. & Young, J. M.(2004). Unexpectedly diverse Mesorhizobium strains and Rhizobium leguminosarum nodulate native legume genera of New Zealand, while introduced legume weeds are nodulated by Bradyrhizobium species. Appl Environ Microbiol 70, 5980–5987.[CrossRef] [Google Scholar]
  33. Willems, A., Doignon-Bourcier, F., Goris, J., Coopman, R., de Lajudie, P., De Vos, P. & Gillis, M.(2001). DNA–DNA hybridization study of Bradyrhizobium strains. Int J Syst Evol Microbiol 51, 1315–1322. [Google Scholar]
  34. Wuertz, S. & Mergeay, M.(1997). Impact of heavy metals on soil microbial communities and their activities. In Modern Soil Microbiology, pp. 607–642. Edited by J. D. Elsas, J. T. Trevors & E. M. H. Wellington. New York: Marcel Dekker.
  35. Yang, Z.(1997).paml: a program package for phylogenetic analysis by maximum likelihood. Comput Appl Biosci 15, 555–556. [Google Scholar]
/content/journal/ijsem/10.1099/ijs.0.003327-0
Loading
Mesorhizobium metallidurans sp. nov., a metal-resistant symbiont of Anthyllis vulneraria growing on metallicolous soil in Languedoc, France
Int J Syst Evol Microbiol 59, 850 (2009); https://doi.org/10.1099/ijs.0.003327-0
/content/journal/ijsem/10.1099/ijs.0.003327-0
/content/journal/ijsem/10.1099/ijs.0.003327-0
Loading

Data & Media loading...

Supplements

vol. , part 4, pp. 850 – 855

GenBank accession numbers for sequences determined in this study [ PDF] (75 KB)

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error