A novel lactic acid bacterium, strain MIC1-18, was isolated from crude oil collected at an oil–water well in Akita, Japan. Cells of strain MIC1-18 were found to be facultatively anaerobic, mesophilic, neutrophilic, Gram-negative, non-sporulating, motile by means of peritrichous flagella and oval rods, 1.8–2.5 μm long. Optimum growth was observed at 30 °C, pH 7.0 and 3 % (w/v) NaCl. Strain MIC1-18 produced acid from -arabinose, ribose, glucose, fructose, mannose, -acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, maltose, sucrose, trehalose, gentiobiose and 5-ketogluconate. -Lactic acid was the major end product from glucose. The major cellular fatty acid was C 7. The cell-wall murein type was A4 containing Lys–Glu. The G+C content of the genomic DNA was 37.8 mol%. Phylogenetic analysis based on the 16S rRNA gene revealed that strain MIC1-18 was accommodated as a member of the lactic acid bacteria of the low-G+C content Gram-positive bacteria; the closest neighbour of this organism was CCUG 48253, with only 90.0 % sequence similarity. On the basis of the phenotypic features and phylogenetic position, a novel genus and species, gen. nov., sp. nov., are proposed for strain MIC1-18 (=NBRC 101988=DSM 19658).


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(a) Phase-contrast and (b) transmission electron micrographs of cells of strain MIC1-18 . Bars, 5.0 µm (a); 0.5 µm (b).



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